酶联生物与您唠唠关于抗体特异性的选择

<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">阳春五月，<span style="color: rgb(255, 0, 0);"><strong>酶联生物</strong></span>与您唠唠关于抗体特异性的挑选。特异性的挑选首要需求考虑四个方面：蛋白特异性、种属特异性、试验办法特异性、符号物的特异性。</span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="color: rgb(255, 102, 0);"><strong><span style="font-family: 宋体; font-size: 9pt;">1<font face="宋体">、蛋白特异性：</font></span></strong></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">针对需求检测的蛋白查找抗体，几个细节要区别，重组表达的蛋白和内源性蛋白的检测，对抗体的要求是不一样的，留意检查抗体阐明书的检测阐明。</span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋体">假如重组蛋白不</font> <font face="宋体">是全长表达，则需求留意抗体的免疫原区域是否在重组蛋白区域内。内源性蛋白能清楚其剪切与润饰的方式，特别表型的蛋白需求进行序列比对，并结合抗体免</font> <font face="宋体">疫原序列，检查穿插反响的状况。磷酸化蛋白检测需求断定详细位点，不同位点的磷酸化意味着可能有不同的机制存在，不宜一概而论。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="color: rgb(255, 102, 0);"><strong><span style="font-family: 宋体; font-size: 9pt;">2<font face="宋体">、种属特异性：</font></span></strong></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋体">同种蛋白不同物种，有着或大或小的差异。现在大部分商品化抗体都是以人源蛋白序列为模板重组蛋白或设计多肽抗原的，依据蛋白的同源状况与其他种属发作穿插</font> <font face="宋体">反响。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋体">需求参照阐明书注明的可反响种属信息。一些稀有种属很难找到抗体，能够经过蛋白的序列比对，挑选同源序列免疫的抗体，不过一般这种状况生产商不会受</font> <font face="宋体">理其关于质量的申述，假如能够申请到免费的抗体样品，则利于抗体挑选。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="color: rgb(255, 102, 0);"><strong><span style="font-family: 宋体; font-size: 9pt;">3<font face="宋体">、试验办法特异性：</font></span></strong></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">现在运用抗体的试验办法有许多，不同的运用办法过程中，由于对蛋白样品的处理方式不一样，蛋白的含量有差异，对抗体的辨认表位和效价要求都是不一样的。</span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋体">详细的依据阐明书注明的产品应用办法进行挑选。可是生产商一般不会将每个抗体都进行各种试验的检测，现在检测比较多的仅仅</font>WB<font face="宋体">、</font><font face="Calibri">IHC</font><font face="宋体">、</font><font face="Calibri">IF</font><font face="宋体">等，假如针对详细 的试验办法没有找到适宜的抗体的，能够结合蛋白序列剖析和抗体的抗原信息，挑选尽可能有作用的抗体。相同，申请到免费的抗体样品是个很好的途径。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="color: rgb(255, 102, 0);"><strong><span style="font-family: 宋体; font-size: 9pt;">4<font face="宋体">、符号物的特异性：</font></span></strong></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋体">一般根据试验操作的试验办法不会运用带有符号的一抗，比方</font>WB<font face="宋体">、</font><font face="Calibri">IHC</font><font face="宋体">等，都是经过二抗类的试剂符号到达成果出现的目的。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋体">以上的内容您把握的怎么样了呢？假如您还有什么不明白的，不妨经过本网站</font>&ldquo;<font face="Calibri">QQ</font><font face="宋体">攀谈&rdquo;联络本公司业务员，咱们将极力为您服务！上海酶联生物一向致力于为广阔高校、科研院所和企事业单位提供一流的科研试剂和完善的技术服务，满意生物化学、分子生物学 、细胞生物学、免疫学等生物科技试验需求。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">&nbsp;</span></p>