丹参酮 A 磺酸钠注射液对兔心肌缺血预处理后血管内皮损伤的保护作用

<p>&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; 刘家军1 吴 松2 李龙珠1 敖金波2<br /> &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;湖北省十堰市中医医院消化科，十堰，442012; 2 十堰市太和医院康复中心，十堰，442000</p> <p><b>&ldquo;上海酶联文献&rdquo;&nbsp;</b><strong>讨论</strong></p> <p>急性心肌梗死 ( Acute Myocardial Infarction，MI) ，中医属心痛、胸痹范畴，其发病主要机制是气虚血瘀，而西药认为在冠状动脉病变如粥样硬化、梗阻的病理基础上导致冠状动脉血供急剧减少或中断 使缺血区心肌严重而持久地急性缺血 。流行病学调查显示急性心肌梗死近年来发病率明显升高，是造成死亡的主要病因［9］，而造成心肌缺血 / 再灌注损伤主要病因是心肌缺血再灌注，其中又以心肌梗死再通手术，冠状动脉溶栓治疗及手术、冠状动脉搭桥手术等最为严重 故缺血前预处理在减轻手术后心肌缺血 / 再灌注损伤的风险方面可能起到极积预防作用。研究表明，VEGF 具有促使血管内皮细胞的增殖、诱导缺血冠状动脉血管新生并增加血管通透性的作用 。而中药制剂丹参酮 A 磺酸钠注射液可扩张冠状动脉血管、增强组织耐缺氧，对缺血 / 再灌注损伤有明确的保护作用 。为探讨丹参酮 A 磺酸钠注射液对急性心肌缺血 / 再灌注损伤预处理后 VEGF 表达及心肌酶谱等的影响，我们从兔耳缘静脉注射丹参酮 A 磺酸钠注射液 7 d 的方法进行缺血预处理来研究其作用机制。</p> <p><br /> &nbsp;</p> <table class="MsoNormalTable" border="0" cellspacing="0" style="border-collapse: collapse; width: 482pt; margin-left: 5.6pt; border-width: initial; border-style: none; border-color: initial;"> <tbody> <tr style="height:22.7000pt;"> <td width="88" valign="bottom" style="width:66.0000pt;padding:0.0000pt 0.0000pt 0.0000pt 0.0000pt ;border-left:none;border-right:none;border-top:none;border-bottom:none;"> <p class="MsoNormal" style="margin-left:56.0000pt;line-height:10.3000pt;mso-line-height-rule:exactly;">&nbsp;</p> </td> <td width="16" valign="bottom" style="width:12.0000pt;padding:0.0000pt 0.0000pt 0.0000pt 0.0000pt ;border-left:none;border-right:none;border-top:none;border-bottom:none;"> <p class="MsoNormal"><span style="font-family:'Times New Roman';font-size:12.0000pt;">&nbsp;</span></p> </td> <td width="40" valign="bottom" style="width:30.0000pt;padding:0.0000pt 0.0000pt 0.0000pt 0.0000pt ;border-left:none;border-right:none;border-top:none;border-bottom:none;"> <p class="MsoNormal" style="margin-left:3.0000pt;line-height:10.3000pt;mso-line-height-rule:exactly;"><span style="mso-spacerun:'yes';font-family:宋体;font-size:9.0000pt;">丹参酮</span><span style="font-family:宋体;font-size:9.0000pt;"><o:p></o:p></span></p> </td> <td width="21" valign="bottom" style="width:16.0000pt;padding:0.0000pt 0.0000pt 0.0000pt 0.0000pt ;border-left:none;border-right:none;border-top:none;border-bottom:none;"> <p class="MsoNormal"><span style="font-family:Arial;font-size:9.0000pt;">A</span><span style="font-family:Arial;font-size:9.0000pt;"><o:p></o:p></span></p> </td> <td width="173" valign="bottom" colspan="4" style="width:130.0000pt;padding:0.0000pt 0.0000pt 0.0000pt 0.0000pt ;border-left:none;border-right:none;border-top:none;border-bottom:none;"> <p class="MsoNormal" align="center" style="text-align:center;line-height:10.3000pt;mso-line-height-rule:exactly;"><span style="mso-spacerun:'yes';font-family:宋体;font-size:9.0000pt;">磺酸钠注射液对兔缺血预处理后</span><span style="font-family:宋体;font-size:9.0000pt;"><o:p></o:p></span></p> </td> <td width="6" valign="bottom" style="width:5.0000pt;padding:0.0000pt 0.0000pt 0.0000pt 0.0000pt ;border-left:none;border-right:none;border-top:none;border-bottom:none;"> <p class="MsoNormal"><span style="font-family:'Times New Roman';font-size:12.0000pt;">&nbsp;</span></p> </td> <td width="40" valign="bottom" style="width:30.0000pt;padding:0.0000pt 0.0000pt 0.0000pt 0.0000pt ;border-left:none;border-right:none;border-top:none;border-bottom:none;"> <p class="MsoNormal"><span style="font-family:'Times New Roman';font-size:12.0000pt;">&nbsp;</span></p> </td> </tr> <tr style="height:8.8000pt;"> <td width="88" valign="bottom" style="width:66.0000pt;padding:0.0000pt 0.0000pt 0.0000pt 0.0000pt ;border-left:none;border-right:none;border-top:none;border-bottom:none;"> <p class="MsoNormal"><span style="font-family:'Times New Roman';font-size:7.5000pt;">&nbsp;</span></p> </td> <td width="16" valign="bottom" style="width:12.0000pt;padding:0.0000pt 0.0000pt 0.0000pt 0.0000pt ;border-left:none;border-right:none;border-top:none;border-bottom:none;"> <p class="MsoNormal" align="right" style="margin-right:2.6000pt;text-align:right;line-height:8.3500pt;mso-line-height-rule:exactly;"><span style="font-family:Arial;font-size:9.0000pt;">3</span><span style="font-family:Arial;font-size:9.0000pt;"><o:p></o:p></span></p> </td> <td width="40" valign="bottom" style="width:30.0000pt;padding:0.0000pt 0.0000pt 0.0000pt 0.0000pt ;border-left:none;border-right:none;border-top:none;border-bottom:none;"> <p class="MsoNormal"><span style="font-family:'Times New Roman';font-size:7.5000pt;">&nbsp;</span></p> </td> <td width="21" valign="bottom" style="width:16.0000pt;padding:0.0000pt 0.0000pt 0.0000pt 0.0000pt ;border-left:none;border-right:none;border-top:none;border-bottom:none;"> <p class="MsoNormal"><span style="font-family:'Times New Roman';font-size:7.5000pt;">&nbsp;</span></p> </td> <td width="110" valign="bottom" style="width:83.0000pt;padding:0.0000pt 0.0000pt 0.0000pt 0.0000pt ;border-left:none;border-right:none;border-top:none;border-bottom:none;"> <p class="MsoNormal"><span style="font-family:'Times New Roman';font-size:7.5000pt;">&nbsp;</span></p> </td> <td width="18" valign="bottom" style="width:14.0000pt;padding:0.0000pt 0.0000pt 0.0000pt 0.0000pt ;border-left:none;border-right:none;border-top:none;border-bottom:none;"> <p class="MsoNormal"><span style="font-family:'Times New Roman';font-size:7.5000pt;">&nbsp;</span></p> </td> <td width="13" valign="bottom" style="width:10.0000pt;padding:0.0000pt 0.0000pt 0.0000pt 0.0000pt ;border-left:none;border-right:none;border-top:none;border-bottom:none;"> <p class="MsoNormal"><span style="font-family:'Times New Roman';font-size:7.5000pt;">&nbsp;</span></p> </td> <td width="30" valign="bottom" style="width:23.0000pt;padding:0.0000pt 0.0000pt 0.0000pt 0.0000pt ;border-left:none;border-right:none;border-top:none;border-bottom:none;"> <p class="MsoNormal"><span style="font-family:'Times New Roman';font-size:7.5000pt;">&nbsp;</span></p> </td> <td width="97" valign="bottom" colspan="3" style="width:73.0000pt;padding:0.0000pt 0.0000pt 0.0000pt 0.0000pt ;border-left:none;border-right:none;border-top:none;border-bottom:none;"> <p class="MsoNormal" style="line-height:8.3500pt;mso-line-height-rule:exactly;"><span style="mso-spacerun:'yes';font-family:Arial;font-size:9.0000pt;">VEGF</span><span style="font-family:'MS PGothic';font-size:9.0000pt;">、</span><span style="mso-spacerun:'yes';font-family:Arial;font-size:9.0000pt;">VEGFm</span><span style="font-family:'MS PGothic';font-size:9.0000pt;">Ｒ</span><span style="mso-spacerun:'yes';font-family:Arial;font-size:9.0000pt;">NA</span><span style="font-family:Arial;font-size:9.0000pt;"><o:p></o:p></span></p> </td> </tr> <tr style="height:4.5000pt;"> <td width="88" valign="bottom" style="width:66.0000pt;padding:0.0000pt 0.0000pt 0.0000pt 0.0000pt ;border-left:none;border-right:none;border-top:none;border-bottom:1.0000pt solid windowtext;mso-border-bottom-alt:1.0000pt solid windowtext;"> <p class="MsoNormal"><span style="font-family:'Times New Roman';font-size:3.5000pt;">&nbsp;</span></p> </td> <td width="16" valign="bottom" style="width:12.0000pt;padding:0.0000pt 0.0000pt 0.0000pt 0.0000pt ;border-left:none;border-right:none;border-top:none;border-bottom:1.0000pt solid windowtext;mso-border-bottom-alt:1.0000pt solid windowtext;"> <p class="MsoNormal"><span style="font-family:'Times New Roman';font-size:3.5000pt;">&nbsp;</span></p> </td> <td width="40" valign="bottom" style="width:30.0000pt;padding:0.0000pt 0.0000pt 0.0000pt 0.0000pt ;border-left:none;border-right:none;border-top:none;border-bottom:1.0000pt solid windowtext;mso-border-bottom-alt:1.0000pt solid windowtext;"> <p class="MsoNormal"><span style="font-family:'Times New Roman';font-size:3.5000pt;">&nbsp;</span></p> </td> <td width="21" valign="bottom" style="width:16.0000pt;padding:0.0000pt 0.0000pt 0.0000pt 0.0000pt ;border-left:none;border-right:none;border-top:none;border-bottom:1.0000pt solid windowtext;mso-border-bottom-alt:1.0000pt solid windowtext;"> <p class="MsoNormal"><span style="font-family:'Times New Roman';font-size:3.5000pt;">&nbsp;</span></p> </td> <td width="129" valign="bottom" colspan="2" style="width:97.0000pt;padding:0.0000pt 0.0000pt 0.0000pt 0.0000pt ;border-left:none;border-right:none;border-top:none;border-bottom:1.0000pt solid windowtext;mso-border-bottom-alt:1.0000pt solid windowtext;"> <p class="MsoNormal"><span style="font-family:'Times New Roman';font-size:3.5000pt;">&nbsp;</span></p> </td> <td width="13" valign="bottom" style="width:10.0000pt;padding:0.0000pt 0.0000pt 0.0000pt 0.0000pt ;border-left:none;border-right:none;border-top:none;border-bottom:1.0000pt solid windowtext;mso-border-bottom-alt:1.0000pt solid windowtext;"> <p class="MsoNormal"><span style="font-family:'Times New 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class="MsoNormal"><span style="font-family:'Times New Roman';font-size:3.5000pt;">&nbsp;</span></p> </td> </tr> <tr style="height:11.1000pt;"> <td width="88" valign="bottom" style="width:66.0000pt;padding:0.0000pt 0.0000pt 0.0000pt 0.0000pt ;border-left:none;border-right:none;border-top:none;border-bottom:none;"> <p class="MsoNormal" align="center" style="margin-left:26.0500pt;text-align:center;line-height:9.1500pt;mso-line-height-rule:exactly;"><span style="mso-spacerun:'yes';font-family:宋体;font-size:8.0000pt;">组别</span><span style="font-family:宋体;font-size:8.0000pt;"><o:p></o:p></span></p> </td> <td width="16" valign="bottom" style="width:12.0000pt;padding:0.0000pt 0.0000pt 0.0000pt 0.0000pt ;border-left:none;border-right:none;border-top:none;border-bottom:none;"> <p class="MsoNormal"><span style="font-family:'Times New Roman';font-size:9.5000pt;">&nbsp;</span></p> </td> <td width="40" valign="bottom" style="width:30.0000pt;padding:0.0000pt 0.0000pt 0.0000pt 0.0000pt ;border-left:none;border-right:none;border-top:none;border-bottom:none;"> <p class="MsoNormal"><span style="font-family:'Times New Roman';font-size:9.5000pt;">&nbsp;</span></p> </td> <td width="21" valign="bottom" style="width:16.0000pt;padding:0.0000pt 0.0000pt 0.0000pt 0.0000pt ;border-left:none;border-right:none;border-top:none;border-bottom:none;"> <p class="MsoNormal"><span style="font-family:'Times New Roman';font-size:9.5000pt;">&nbsp;</span></p> </td> <td width="129" valign="bottom" colspan="2" style="width:97.0000pt;padding:0.0000pt 0.0000pt 0.0000pt 0.0000pt ;border-left:none;border-right:none;border-top:none;border-bottom:1.0000pt solid windowtext;mso-border-bottom-alt:1.0000pt solid windowtext;"> <p class="MsoNormal" align="center" style="margin-left:30.8500pt;text-align:center;line-height:9.7000pt;mso-line-height-rule:exactly;"><span style="mso-spacerun:'yes';font-family:Arial;font-size:8.0000pt;">VEGF</span><span style="font-family:宋体;font-size:8.0000pt;">(</span><span style="mso-spacerun:'yes';font-family:Arial;font-size:8.0000pt;">&nbsp;Pg / mL</span><span style="font-family:宋体;font-size:8.0000pt;">)</span><span style="font-family:宋体;font-size:8.0000pt;"><o:p></o:p></span></p> </td> <td width="13" valign="bottom" style="width:10.0000pt;padding:0.0000pt 0.0000pt 0.0000pt 0.0000pt ;border-left:none;border-right:none;border-top:none;border-bottom:1.0000pt solid windowtext;mso-border-bottom-alt:1.0000pt solid windowtext;"> <p class="MsoNormal"><span style="font-family:'Times New Roman';font-size:9.5000pt;">&nbsp;</span></p> </td> <td width="30" valign="bottom" style="width:23.0000pt;padding:0.0000pt 0.0000pt 0.0000pt 0.0000pt ;border-left:none;border-right:none;border-top:none;border-bottom:1.0000pt solid windowtext;mso-border-bottom-alt:1.0000pt solid windowtext;"> <p class="MsoNormal"><span style="font-family:'Times New Roman';font-size:9.5000pt;">&nbsp;</span></p> </td> <td width="6" valign="bottom" style="width:5.0000pt;padding:0.0000pt 0.0000pt 0.0000pt 0.0000pt ;border-left:none;border-right:none;border-top:none;border-bottom:1.0000pt solid windowtext;mso-border-bottom-alt:1.0000pt solid windowtext;"> <p class="MsoNormal"><span style="font-family:'Times New Roman';font-size:9.5000pt;">&nbsp;</span></p> </td> <td width="40" valign="bottom" style="width:30.0000pt;padding:0.0000pt 0.0000pt 0.0000pt 0.0000pt ;border-left:none;border-right:none;border-top:none;border-bottom:none;"> <p class="MsoNormal"><span style="font-family:'Times New Roman';font-size:9.5000pt;">&nbsp;</span></p> </td> </tr> <tr style="height:2.3500pt;"> <td width="88" valign="bottom" style="width:66.0000pt;padding:0.0000pt 0.0000pt 0.0000pt 0.0000pt ;border-left:none;border-right:none;border-top:none;border-bottom:none;"> <p class="MsoNormal"><span style="font-family:'Times New Roman';font-size:2.0000pt;">&nbsp;</span></p> </td> <td width="16" valign="bottom" style="width:12.0000pt;padding:0.0000pt 0.0000pt 0.0000pt 0.0000pt ;border-left:none;border-right:none;border-top:none;border-bottom:none;"> <p class="MsoNormal"><span style="font-family:'Times New Roman';font-size:2.0000pt;">&nbsp;</span></p> </td> <td width="40" valign="bottom" style="width:30.0000pt;padding:0.0000pt 0.0000pt 0.0000pt 0.0000pt ;border-left:none;border-right:none;border-top:none;border-bottom:none;"> <p class="MsoNormal"><span style="font-family:'Times New Roman';font-size:2.0000pt;">&nbsp;</span></p> </td> <td width="21" valign="bottom" style="width:16.0000pt;padding:0.0000pt 0.0000pt 0.0000pt 0.0000pt ;border-left:none;border-right:none;border-top:none;border-bottom:none;"> <p class="MsoNormal"><span style="font-family:'Times New Roman';font-size:2.0000pt;">&nbsp;</span></p> </td> <td width="110" valign="bottom" style="width:83.0000pt;padding:0.0000pt 0.0000pt 0.0000pt 0.0000pt ;border-left:none;border-right:none;border-top:none;border-bottom:none;"> <p class="MsoNormal" align="right" style="margin-right:31.8500pt;text-align:right;line-height:8.3000pt;mso-line-height-rule:exactly;"><span style="mso-spacerun:'yes';font-family:宋体;font-size:8.0000pt;">缺血预处理前</span><span style="font-family:宋体;font-size:8.0000pt;"><o:p></o:p></span></p> </td> <td width="109" valign="bottom" colspan="5" style="width:82.0000pt;padding:0.0000pt 0.0000pt 0.0000pt 0.0000pt ;border-left:none;border-right:none;border-top:none;border-bottom:none;"> <p class="MsoNormal" style="margin-left:4.0000pt;line-height:8.3000pt;mso-line-height-rule:exactly;"><span style="mso-spacerun:'yes';font-family:宋体;font-size:8.0000pt;">缺血预处理后</span><span style="font-family:宋体;font-size:8.0000pt;"><o:p></o:p></span></p> </td> </tr> <tr style="height:5.9500pt;"> <td width="88" valign="bottom" style="width:66.0000pt;padding:0.0000pt 0.0000pt 0.0000pt 0.0000pt ;border-left:none;border-right:none;border-top:none;border-bottom:none;"> <p class="MsoNormal"><span style="font-family:'Times New 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style="width:14.0000pt;padding:0.0000pt 0.0000pt 0.0000pt 0.0000pt ;border-left:none;border-right:none;border-top:none;border-bottom:1.0000pt solid windowtext;mso-border-bottom-alt:1.0000pt solid windowtext;"> <p class="MsoNormal"><span style="font-family:'Times New Roman';font-size:2.0000pt;">&nbsp;</span></p> </td> <td width="13" valign="bottom" style="width:10.0000pt;padding:0.0000pt 0.0000pt 0.0000pt 0.0000pt ;border-left:none;border-right:none;border-top:none;border-bottom:1.0000pt solid windowtext;mso-border-bottom-alt:1.0000pt solid windowtext;"> <p class="MsoNormal"><span style="font-family:'Times New Roman';font-size:2.0000pt;">&nbsp;</span></p> </td> <td width="30" valign="bottom" style="width:23.0000pt;padding:0.0000pt 0.0000pt 0.0000pt 0.0000pt ;border-left:none;border-right:none;border-top:none;border-bottom:1.0000pt solid windowtext;mso-border-bottom-alt:1.0000pt solid windowtext;"> <p class="MsoNormal"><span style="font-family:'Times New Roman';font-size:2.0000pt;">&nbsp;</span></p> </td> <td width="6" valign="bottom" style="width:5.0000pt;padding:0.0000pt 0.0000pt 0.0000pt 0.0000pt ;border-left:none;border-right:none;border-top:none;border-bottom:1.0000pt solid windowtext;mso-border-bottom-alt:1.0000pt solid windowtext;"> <p class="MsoNormal"><span style="font-family:'Times New Roman';font-size:2.0000pt;">&nbsp;</span></p> </td> <td width="40" valign="bottom" style="width:30.0000pt;padding:0.0000pt 0.0000pt 0.0000pt 0.0000pt ;border-left:none;border-right:none;border-top:none;border-bottom:1.0000pt solid windowtext;mso-border-bottom-alt:1.0000pt solid windowtext;"> <p class="MsoNormal"><span style="font-family:'Times New Roman';font-size:2.0000pt;">&nbsp;</span></p> </td> </tr> <tr style="height:9.6000pt;"> <td width="88" valign="bottom" style="width:66.0000pt;padding:0.0000pt 0.0000pt 0.0000pt 0.0000pt ;border-left:none;border-right:none;border-top:none;border-bottom:none;"> <p class="MsoNormal" align="center" style="margin-left:26.0500pt;text-align:center;line-height:8.8500pt;mso-line-height-rule:exactly;"><span style="mso-spacerun:'yes';font-family:宋体;font-size:8.0000pt;">对照组</span><span style="font-family:宋体;font-size:8.0000pt;"><o:p></o:p></span></p> </td> <td width="16" valign="bottom" style="width:12.0000pt;padding:0.0000pt 0.0000pt 0.0000pt 0.0000pt ;border-left:none;border-right:none;border-top:none;border-bottom:none;"> <p class="MsoNormal"><span style="font-family:'Times New Roman';font-size:8.0000pt;">&nbsp;</span></p> </td> <td width="40" valign="bottom" style="width:30.0000pt;padding:0.0000pt 0.0000pt 0.0000pt 0.0000pt ;border-left:none;border-right:none;border-top:none;border-bottom:none;"> <p class="MsoNormal"><span style="font-family:'Times New Roman';font-size:8.0000pt;">&nbsp;</span></p> </td> <td width="132" valign="bottom" colspan="2" style="width:99.0000pt;padding:0.0000pt 0.0000pt 0.0000pt 0.0000pt ;border-left:none;border-right:none;border-top:none;border-bottom:none;"> <p class="MsoNormal" align="right" style="margin-right:37.8500pt;text-align:right;mso-line-height-alt:0pt;"><span style="mso-spacerun:'yes';font-family:Arial;font-size:8.0000pt;">56. 24 &plusmn; 4. 27</span><span style="font-family:Arial;font-size:8.0000pt;"><o:p></o:p></span></p> </td> <td width="18" valign="bottom" style="width:14.0000pt;padding:0.0000pt 0.0000pt 0.0000pt 0.0000pt ;border-left:none;border-right:none;border-top:none;border-bottom:none;"> <p class="MsoNormal" align="right" style="text-align:right;mso-line-height-alt:0pt;"><span style="mso-spacerun:'yes';font-family:Arial;font-size:8.0000pt;">54.</span><span style="font-family:Arial;font-size:8.0000pt;"><o:p></o:p></span></p> </td> <td width="13" valign="bottom" style="width:10.0000pt;padding:0.0000pt 0.0000pt 0.0000pt 0.0000pt ;border-left:none;border-right:none;border-top:none;border-bottom:none;"> <p class="MsoNormal" align="right" style="text-align:right;mso-line-height-alt:0pt;"><span style="mso-spacerun:'yes';font-family:Arial;font-size:8.0000pt;">59</span><span style="font-family:Arial;font-size:8.0000pt;"><o:p></o:p></span></p> </td> <td width="30" valign="bottom" style="width:23.0000pt;padding:0.0000pt 0.0000pt 0.0000pt 0.0000pt ;border-left:none;border-right:none;border-top:none;border-bottom:none;"> <p class="MsoNormal" style="margin-left:2.0000pt;mso-line-height-alt:0pt;"><span style="mso-spacerun:'yes';font-family:Arial;font-size:8.0000pt;">&plusmn; 5. 39</span><span style="font-family:Arial;font-size:8.0000pt;"><o:p></o:p></span></p> </td> <td width="6" valign="bottom" style="width:5.0000pt;padding:0.0000pt 0.0000pt 0.0000pt 0.0000pt ;border-left:none;border-right:none;border-top:none;border-bottom:none;"> <p class="MsoNormal"><span style="font-family:'Times New Roman';font-size:8.0000pt;">&nbsp;</span></p> </td> <td width="40" valign="bottom" style="width:30.0000pt;padding:0.0000pt 0.0000pt 0.0000pt 0.0000pt ;border-left:none;border-right:none;border-top:none;border-bottom:none;"> <p class="MsoNormal"><span style="font-family:'Times New Roman';font-size:8.0000pt;">&nbsp;</span></p> </td> </tr> <tr style="height:13.6500pt;"> <td width="104" valign="bottom" colspan="2" style="width:78.0000pt;padding:0.0000pt 0.0000pt 0.0000pt 0.0000pt ;border-left:none;border-right:none;border-top:none;border-bottom:none;"> <p class="MsoNormal" align="center" style="margin-left:14.0500pt;text-align:center;line-height:9.7000pt;mso-line-height-rule:exactly;"><span style="mso-spacerun:'yes';font-family:宋体;font-size:8.0000pt;">缺血</span><span style="mso-spacerun:'yes';font-family:Arial;font-size:8.0000pt;">&nbsp;/ </span><span style="mso-spacerun:'yes';font-family:宋体;font-size:8.0000pt;">再灌注组</span><span style="font-family:宋体;font-size:8.0000pt;"><o:p></o:p></span></p> </td> <td width="40" valign="bottom" style="width:30.0000pt;padding:0.0000pt 0.0000pt 0.0000pt 0.0000pt ;border-left:none;border-right:none;border-top:none;border-bottom:none;"> <p class="MsoNormal"><span style="font-family:'Times New Roman';font-size:11.5000pt;">&nbsp;</span></p> </td> <td width="132" valign="bottom" colspan="2" style="width:99.0000pt;padding:0.0000pt 0.0000pt 0.0000pt 0.0000pt ;border-left:none;border-right:none;border-top:none;border-bottom:none;"> <p class="MsoNormal" align="right" style="margin-right:37.8500pt;text-align:right;mso-line-height-alt:0pt;"><span style="mso-spacerun:'yes';font-family:Arial;font-size:8.0000pt;">55. 72 &plusmn; 3. 59</span><span style="font-family:Arial;font-size:8.0000pt;"><o:p></o:p></span></p> </td> <td width="18" valign="bottom" style="width:14.0000pt;padding:0.0000pt 0.0000pt 0.0000pt 0.0000pt ;border-left:none;border-right:none;border-top:none;border-bottom:none;"> <p class="MsoNormal" align="right" style="text-align:right;mso-line-height-alt:0pt;"><span style="mso-spacerun:'yes';font-family:Arial;font-size:8.0000pt;">79.</span><span style="font-family:Arial;font-size:8.0000pt;"><o:p></o:p></span></p> </td> <td width="13" valign="bottom" style="width:10.0000pt;padding:0.0000pt 0.0000pt 0.0000pt 0.0000pt ;border-left:none;border-right:none;border-top:none;border-bottom:none;"> <p class="MsoNormal" align="right" style="text-align:right;mso-line-height-alt:0pt;"><span style="mso-spacerun:'yes';font-family:Arial;font-size:8.0000pt;">86</span><span style="font-family:Arial;font-size:8.0000pt;"><o:p></o:p></span></p> </td> <td width="37" valign="bottom" colspan="2" style="width:28.0000pt;padding:0.0000pt 0.0000pt 0.0000pt 0.0000pt ;border-left:none;border-right:none;border-top:none;border-bottom:none;"> <p class="MsoNormal" style="margin-left:2.0000pt;mso-line-height-alt:0pt;"><span style="mso-spacerun:'yes';font-family:Arial;font-size:8.0000pt;">&plusmn; 7. 31</span>^{<span style="font-family:Arial;vertical-align:super;">*</span>}^{<span style="font-family:Arial;vertical-align:super;"><o:p></o:p></span>}</p> </td> <td width="40" valign="bottom" style="width:30.0000pt;padding:0.0000pt 0.0000pt 0.0000pt 0.0000pt ;border-left:none;border-right:none;border-top:none;border-bottom:none;"> <p class="MsoNormal"><span style="font-family:'Times New Roman';font-size:11.5000pt;">&nbsp;</span></p> </td> </tr> <tr style="height:11.9500pt;"> <td width="104" valign="bottom" colspan="2" style="width:78.0000pt;padding:0.0000pt 0.0000pt 0.0000pt 0.0000pt ;border-left:none;border-right:none;border-top:none;border-bottom:none;"> <p class="MsoNormal" style="margin-left:20.0000pt;line-height:9.7000pt;mso-line-height-rule:exactly;"><span style="mso-spacerun:'yes';font-family:宋体;font-size:8.0000pt;">缺血预处理</span><span style="mso-spacerun:'yes';font-family:Arial;font-size:8.0000pt;">&nbsp;A </span><span style="font-family:宋体;font-size:8.0000pt;">组</span><span style="font-family:宋体;font-size:8.0000pt;"><o:p></o:p></span></p> </td> <td width="40" valign="bottom" style="width:30.0000pt;padding:0.0000pt 0.0000pt 0.0000pt 0.0000pt ;border-left:none;border-right:none;border-top:none;border-bottom:none;"> <p class="MsoNormal"><span style="font-family:'Times New Roman';">&nbsp;</span></p> </td> <td width="132" valign="bottom" colspan="2" style="width:99.0000pt;padding:0.0000pt 0.0000pt 0.0000pt 0.0000pt ;border-left:none;border-right:none;border-top:none;border-bottom:none;"> <p class="MsoNormal" align="right" style="margin-right:37.8500pt;text-align:right;mso-line-height-alt:0pt;"><span style="mso-spacerun:'yes';font-family:Arial;font-size:8.0000pt;">57. 05 &plusmn; 4. 75</span><span style="font-family:Arial;font-size:8.0000pt;"><o:p></o:p></span></p> </td> <td width="18" valign="bottom" style="width:14.0000pt;padding:0.0000pt 0.0000pt 0.0000pt 0.0000pt ;border-left:none;border-right:none;border-top:none;border-bottom:none;"> <p class="MsoNormal" align="right" style="text-align:right;mso-line-height-alt:0pt;"><span style="mso-spacerun:'yes';font-family:Arial;font-size:8.0000pt;">104.</span><span style="font-family:Arial;font-size:8.0000pt;"><o:p></o:p></span></p> </td> <td width="13" valign="bottom" style="width:10.0000pt;padding:0.0000pt 0.0000pt 0.0000pt 0.0000pt ;border-left:none;border-right:none;border-top:none;border-bottom:none;"> <p class="MsoNormal" align="right" style="text-align:right;mso-line-height-alt:0pt;"><span style="mso-spacerun:'yes';font-family:Arial;font-size:8.0000pt;">59</span><span style="font-family:Arial;font-size:8.0000pt;"><o:p></o:p></span></p> </td> <td width="77" valign="bottom" colspan="3" style="width:58.0000pt;padding:0.0000pt 0.0000pt 0.0000pt 0.0000pt ;border-left:none;border-right:none;border-top:none;border-bottom:none;"> <p class="MsoNormal" style="margin-left:1.0000pt;line-height:8.9000pt;mso-line-height-rule:exactly;"><span style="mso-spacerun:'yes';font-family:Arial;font-size:8.0000pt;">&plusmn; 9. 24</span><span style="font-family:Arial;font-size:8.0000pt;"><o:p></o:p></span></p> </td> </tr> <tr style="height:11.3500pt;"> <td width="104" valign="bottom" colspan="2" style="width:78.0000pt;padding:0.0000pt 0.0000pt 0.0000pt 0.0000pt ;border-left:none;border-right:none;border-top:none;border-bottom:1.0000pt solid windowtext;mso-border-bottom-alt:1.0000pt solid windowtext;"> <p class="MsoNormal" style="margin-left:20.0000pt;line-height:9.7000pt;mso-line-height-rule:exactly;"><span style="mso-spacerun:'yes';font-family:宋体;font-size:8.0000pt;">缺血预处理</span><span style="mso-spacerun:'yes';font-family:Arial;font-size:8.0000pt;">&nbsp;B </span><span style="font-family:宋体;font-size:8.0000pt;">组</span><span style="font-family:宋体;font-size:8.0000pt;"><o:p></o:p></span></p> </td> <td width="40" valign="bottom" style="width:30.0000pt;padding:0.0000pt 0.0000pt 0.0000pt 0.0000pt ;border-left:none;border-right:none;border-top:none;border-bottom:1.0000pt solid windowtext;mso-border-bottom-alt:1.0000pt solid windowtext;"> <p class="MsoNormal"><span style="font-family:'Times New Roman';font-size:9.5000pt;">&nbsp;</span></p> </td> <td width="132" valign="bottom" colspan="2" style="width:99.0000pt;padding:0.0000pt 0.0000pt 0.0000pt 0.0000pt ;border-left:none;border-right:none;border-top:none;border-bottom:1.0000pt solid windowtext;mso-border-bottom-alt:1.0000pt solid windowtext;"> <p class="MsoNormal" align="right" style="margin-right:37.8500pt;text-align:right;mso-line-height-alt:0pt;"><span style="mso-spacerun:'yes';font-family:Arial;font-size:8.0000pt;">58. 47 &plusmn; 4. 73</span><span style="font-family:Arial;font-size:8.0000pt;"><o:p></o:p></span></p> </td> <td width="18" valign="bottom" style="width:14.0000pt;padding:0.0000pt 0.0000pt 0.0000pt 0.0000pt ;border-left:none;border-right:none;border-top:none;border-bottom:1.0000pt solid windowtext;mso-border-bottom-alt:1.0000pt solid windowtext;"> <p class="MsoNormal" align="right" style="text-align:right;mso-line-height-alt:0pt;"><span style="mso-spacerun:'yes';font-family:Arial;font-size:8.0000pt;">107.</span><span style="font-family:Arial;font-size:8.0000pt;"><o:p></o:p></span></p> </td> <td width="13" valign="bottom" style="width:10.0000pt;padding:0.0000pt 0.0000pt 0.0000pt 0.0000pt ;border-left:none;border-right:none;border-top:none;border-bottom:1.0000pt solid windowtext;mso-border-bottom-alt:1.0000pt solid windowtext;"> <p class="MsoNormal" align="right" style="text-align:right;mso-line-height-alt:0pt;"><span style="mso-spacerun:'yes';font-family:Arial;font-size:8.0000pt;">97</span><span style="font-family:Arial;font-size:8.0000pt;"><o:p></o:p></span></p> </td> <td width="77" valign="bottom" colspan="3" style="width:58.0000pt;padding:0.0000pt 0.0000pt 0.0000pt 0.0000pt ;border-left:none;border-right:none;border-top:none;border-bottom:1.0000pt solid windowtext;mso-border-bottom-alt:1.0000pt solid windowtext;"> <p class="MsoNormal" style="margin-left:1.0000pt;line-height:8.3000pt;mso-line-height-rule:exactly;"><span style="mso-spacerun:'yes';font-family:Arial;font-size:8.0000pt;">&plusmn; 10. 48</span><span style="font-family:Arial;font-size:8.0000pt;"><o:p></o:p></span></p> </td> </tr> <tr style="height:9.3000pt;"> <td width="88" valign="bottom" style="width:66.0000pt;padding:0.0000pt 0.0000pt 0.0000pt 0.0000pt ;border-left:none;border-right:none;border-top:none;border-bottom:none;"> <p class="MsoNormal"><span style="font-family:'Times New Roman';font-size:8.0000pt;">&nbsp;</span></p> </td> <td width="16" valign="bottom" style="width:12.0000pt;padding:0.0000pt 0.0000pt 0.0000pt 0.0000pt ;border-left:none;border-right:none;border-top:none;border-bottom:none;"> <p class="MsoNormal"><span style="font-family:'Times New Roman';font-size:8.0000pt;">&nbsp;</span></p> </td> <td width="40" valign="bottom" style="width:30.0000pt;padding:0.0000pt 0.0000pt 0.0000pt 0.0000pt ;border-left:none;border-right:none;border-top:none;border-bottom:none;"> <p class="MsoNormal" style="margin-left:2.0000pt;mso-line-height-alt:0pt;"><span style="font-family:Arial;font-size:5.0000pt;">*</span><span style="font-family:Arial;font-size:5.0000pt;"><o:p></o:p></span></p> </td> <td width="21" valign="bottom" style="width:16.0000pt;padding:0.0000pt 0.0000pt 0.0000pt 0.0000pt ;border-left:none;border-right:none;border-top:none;border-bottom:none;"> <p class="MsoNormal"><span style="font-family:'Times New Roman';font-size:8.0000pt;">&nbsp;</span></p> </td> <td width="110" valign="bottom" style="width:83.0000pt;padding:0.0000pt 0.0000pt 0.0000pt 0.0000pt ;border-left:none;border-right:none;border-top:none;border-bottom:none;"> <p class="MsoNormal"><span style="font-family:'Times New Roman';font-size:8.0000pt;">&nbsp;</span></p> </td> <td width="18" valign="bottom" style="width:14.0000pt;padding:0.0000pt 0.0000pt 0.0000pt 0.0000pt ;border-left:none;border-right:none;border-top:none;border-bottom:none;"> <p class="MsoNormal"><span style="font-family:'Times New Roman';font-size:8.0000pt;">&nbsp;</span></p> </td> <td width="13" valign="bottom" style="width:10.0000pt;padding:0.0000pt 0.0000pt 0.0000pt 0.0000pt ;border-left:none;border-right:none;border-top:none;border-bottom:none;"> <p class="MsoNormal"><span style="font-family:'Times New Roman';font-size:8.0000pt;">&nbsp;</span></p> </td> <td width="30" valign="bottom" style="width:23.0000pt;padding:0.0000pt 0.0000pt 0.0000pt 0.0000pt ;border-left:none;border-right:none;border-top:none;border-bottom:none;"> <p class="MsoNormal"><span style="font-family:'Times New Roman';font-size:8.0000pt;">&nbsp;</span></p> </td> <td width="6" valign="bottom" style="width:5.0000pt;padding:0.0000pt 0.0000pt 0.0000pt 0.0000pt ;border-left:none;border-right:none;border-top:none;border-bottom:none;"> <p class="MsoNormal"><span style="font-family:'Times New Roman';font-size:8.0000pt;">&nbsp;</span></p> </td> <td width="40" valign="bottom" style="width:30.0000pt;padding:0.0000pt 0.0000pt 0.0000pt 0.0000pt ;border-left:none;border-right:none;border-top:none;border-bottom:none;"> <p class="MsoNormal"><span style="font-family:'Times New Roman';font-size:8.0000pt;">&nbsp;</span></p> </td> </tr> <tr style="height:9.2000pt;"> <td width="338" valign="bottom" colspan="8" style="width:254.0000pt;padding:0.0000pt 0.0000pt 0.0000pt 0.0000pt ;border-left:none;border-right:none;border-top:none;border-bottom:none;"> <p class="MsoNormal" style="margin-left:16.0000pt;line-height:9.2500pt;mso-line-height-rule:exactly;"><span style="mso-spacerun:'yes';font-family:宋体;font-size:8.0000pt;">注: 与对照组比较，</span><span style="mso-spacerun:'yes';font-family:Arial;font-size:8.0000pt;">&nbsp;P </span><span style="font-family:'MS PGothic';font-size:8.0000pt;">＜</span><span style="mso-spacerun:'yes';font-family:Arial;font-size:8.0000pt;">&nbsp;0. 05</span><span style="font-family:宋体;font-size:8.0000pt;">;</span><span style="font-family:Arial;font-size:8.0000pt;">&nbsp;</span><span style="mso-spacerun:'yes';font-family:宋体;font-size:8.0000pt;">与缺血</span><span style="mso-spacerun:'yes';font-family:Arial;font-size:8.0000pt;">&nbsp;/ </span><span style="mso-spacerun:'yes';font-family:宋体;font-size:8.0000pt;">再灌注组比较，</span><span style="mso-spacerun:'yes';font-family:Arial;font-size:8.0000pt;">&nbsp;P </span><span style="font-family:'MS PGothic';font-size:8.0000pt;">＜</span><span style="mso-spacerun:'yes';font-family:Arial;font-size:8.0000pt;">&nbsp;0. 05</span><span style="font-family:'MS PGothic';font-size:8.0000pt;">。</span><span style="font-family:'MS PGothic';font-size:8.0000pt;"><o:p></o:p></span></p> </td> <td width="6" valign="bottom" style="width:5.0000pt;padding:0.0000pt 0.0000pt 0.0000pt 0.0000pt ;border-left:none;border-right:none;border-top:none;border-bottom:none;"> <p class="MsoNormal"><span style="font-family:'Times New Roman';font-size:8.0000pt;">&nbsp;</span></p> </td> <td width="40" valign="bottom" style="width:30.0000pt;padding:0.0000pt 0.0000pt 0.0000pt 0.0000pt ;border-left:none;border-right:none;border-top:none;border-bottom:none;"> <p class="MsoNormal"><span style="font-family:'Times New Roman';font-size:8.0000pt;"><o:p></o:p></span></p> </td> </tr> </tbody> </table> <p>&nbsp;</p> <p>现代医学证实，在正常生理代谢状态下，机体中氧自由基生成与清除及血管内皮功能均维持着动态平衡，当机体发生病理改变如心肌组织由于缺血 / 再灌注时，组织缺血缺氧会造成清除自由基的酶 ( 总超氧化物歧化酶) 活性下降，脂质过氧化反应加剧造成血管内皮细胞严重损伤，此时心肌酶谱明显异常，如血清 T-SOD、LDH 及 CK-MB 水平在缺血预处理后明显降低，MDA 大量释放于血液而导致异常增高，是 诊 断 心 肌 敏 感 度 和 特 异 度 较 高&ldquo;金标准&rdquo;。在缺血 / 再灌注中 VEGF 作为一种高度特异性的促血管生长因子也因缺血刺激而出现增高以弥补心肌缺血缺氧。由于 VEGF 具有促进血管内皮细胞增殖迁移再生、诱导多种血管活性物质合成。</p> <p>&nbsp;</p>