核酸适配体在真菌毒素检测中研究进展

<p>&nbsp;</p> <p><strong>【摘 要】</strong>核酸适配体是指在体外利用指数富集的配基系统进化技术筛选得到的核酸（DNA 或 RNA）片段。基于核酸适配体的检测方法具有特异性高、稳定性好、易于修饰等优点，在真菌毒素检测领域得到了广泛的关注和应用。本文在传统检测方法的基础上，着重介绍核酸适配体在真菌毒素检测领域中的研究进展，并提出目前存在的问题及发展趋势。&nbsp;</p> <p><img src="/images/upload/Image/4444(1).png" width="694" height="637" alt="" /></p> <p><b>&ldquo;上海酶联&rdquo;&nbsp;</b><strong>参考文献</strong></p> <p>[ 1 ] 张思思, 陆继伟, 王少敏,等. 国内外真菌毒素检测方法研究现状及进展[J]. 食品安全质量检测学报, 2016, 7(7): 2575 &ndash; 2586.</p> <p>[ 2 ] International Agency for Research on Cancer. IARC monographs on the evaluation of carcinogenic risks to humans, 2015 [EB/OL]. Available from: http://monographs.Iarc.fr/ENG/Classification/ latest_classif.php.</p> <p>[ 3 ] 王元凯, 王君, 严亚贤. 玉米赤霉烯酮检测方法研究进展[J]. 中国公共卫生, 2009, 25(9): 1100 &ndash; 1101.</p> <p>[ 4 ] 李蓉, 黄莹偲, 王勇,等. 食品中真菌毒素检测技术的研究进展[J].中国卫生检验杂志, 2015, 25(18): 3195 &ndash; 3198.</p> <p>[ 5 ] Jutamart K, Saranya P, Supaporn I, et al. Simultaneous detection of multiple mycotoxins in broiler feeds using a liquid chromatography tandem-mass spectrometry[J]. Journal of Veterinary Medical Science, 2015, 78(2): 259 &ndash; 264.</p> <p>[ 6 ] Tuerk C, Gold L. Systematic evolution of ligands by exponential enrichment: RNA ligands to bacteriophage T4 DNA polymerase[J]. Science, 1990, 249(4968): 505 &ndash; 510.</p> <p>[ 7 ] Ellington AD, Szostak JW. In vitro selection of RNA molecules that bind specific ligands[J]. Nature, 1990, 346(6287): 818 &ndash; 822.</p> <p>[ 8 ] Y&uuml;ce M, Ullah N, Budak H. Trends in aptamer selection methods and applications[J]. Analyst, 2015, 140(16): 5379 &ndash; 5399.</p> <p>[ 9 ] Cruzaguado JA, Penner G. Determination of ochratoxin a with a DNA aptamer[J]. Journal of Agricultural and Food Chemistry, 2017, 56(22): 10456 &ndash; 10461.</p> <p>[ 10 ] Mckeague M, Velu R, Hill K, et al. Selection and characterization of a novel DNA aptamer for label-free fluorescence biosensing of ochratoxin A[J]. Toxins, 2014, 6(8): 2435 &ndash; 2452.</p> <p>[ 11 ] Barthelmebs L, Jonca J, Hayat A, et al. Enzyme-linked aptamer assays (ELAAs), based on a competition format for a rapid and sensitive detection of ochratoxin A in wine[J]. Food Control, 2011, 22(5): 737 &ndash; 743.</p> <p>[ 12 ] Le LC, Cruz-Aguado JA, Penner GA. Ligands for aflatoxin and zearalenone: US, WO/2011/020198[P]. 2011.</p> <p>[ 13 ] Ma X, Wang W, Chen X, et al. Selection, identification, and application of aflatoxin B1 aptamer[J]. European Food Research and Technology, 2014, 238(6): 919 &ndash; 925.</p> <p>[ 14 ] Setlem K, Mondal B, Ramlal S, et al. Immuno affinity SELEX for simple, rapid, and cost-effective aptamer enrichment and identification against aflatoxin B1[J]. Frontiers in Microbiology, 2016, 7: 1909.</p> <p>[ 15 ] Ma X, Wang W, Chen X, et al. Selection, characterization and application of aptamers targeted to aflatoxin B2[J]. Food Control, 2015, 47: 545 &ndash; 551.</p> <p>[ 16 ] Malhotra S, Pandey AK, Rajput YS, et al. Selection of aptamers for aflatoxin M1 and their characterization[J]. Journal of Molecular Recognition, 2014, 27(8): 493 &ndash; 500.</p> <p>[ 17 ] Mckeague M, Bradley CR, Girolamo AD, et al. Screening and initial binding assessment of fumonisin B1 aptamers[J]. International Journal of Molecular Sciences, 2010, 11(12): 4864 &ndash; 4881.</p> <p>[ 18 ] Chen X, Huang Y, Duan N, et al. Selection and characterization of single stranded DNA aptamers recognizing fumonisin B1[J]. Microchimica Acta, 2014, 181(11): 1317 &ndash; 1324.</p> <p>[ 19 ] Frost NR, Mckeague M, Falcioni D, et al. An in solution assay for interrogation of affinity and rational minimer design for small molecule-binding aptamers[J]. Analyst, 2015, 140(19): 6643 &ndash; 6651.</p> <p>[ 20 ] Chen XJ, Huang YK, Duan N, et al. Selection and identification of ssDNA aptamers recognizing zearalenone[J]. Analytical and Bioanalytical Chemistry, 2013, 405(20): 6573 &ndash; 6581.</p> <p>[ 21 ] Chen XJ, Huang YK, Duan N, et al. Screening and identification of DNA aptamers against T-2 toxin assisted by graphene</p> <p>&nbsp;</p>