别让ELISA实验功亏一篑！从原理到选购，一文教你读懂酶标仪

<p>&nbsp;<span style="font-family: 宋体; font-size: 10.5pt;">&nbsp;&nbsp;&nbsp;&nbsp;</span><font face="Calibri" style="font-size: 10.5pt;">ELISA</font><font face="宋体" style="font-family: 宋体; font-size: 10.5pt;">实验的最后一步，决不能功亏一篑！还在担心酶标仪的突然罢工，让您数小时的心血、珍贵的样品和试剂瞬间化为泡影吗？一台真正可靠的酶标仪，是您获得精准数据的最后一道保障。它，究竟该是什么样？</font></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">&nbsp;&nbsp;&nbsp;&nbsp;<font face="宋体">酶标仪，即酶联免疫检测仪，用于检测</font><font face="Calibri">ELISA</font><font face="宋体">反应后的吸光度值。它主要由光路系统和信号采集系统组成。其工作原理为：</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">&nbsp; &nbsp; 1、<font face="宋体">光源灯发出的光波经过滤光片或单色器转变为一束单色光，一部分单色光被标本吸收，另一部分透射过标本照射到光电探测器上；</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">&nbsp; &nbsp; 2、<font face="宋体">光电探测器将光信号转换成相应的电信号，经过一系列的信号处理后，送入微处理器进行数据处理和计算，最后将结果显示出来。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">&nbsp;&nbsp;&nbsp;&nbsp;<strong><font face="宋体">微孔板读数仪分类</font></strong></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">&nbsp;&nbsp;&nbsp;&nbsp;<font face="宋体">根据光学系统的类型，酶标仪可分为滤光片式酶标仪和光栅式酶标仪。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">&nbsp;&nbsp;&nbsp;&nbsp;<font face="宋体">光学滤光片是一块涂有光学薄膜的光学玻璃板，它可以改变通过滤光片的波长，并分离不需要的波长。根据选配的滤光片，可以仅截取特定波长进行检测。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">&nbsp;&nbsp;&nbsp;&nbsp;<font face="宋体">光栅式是利用物理衍射光栅的原理，将白光源以不同的角度分光，再通过一系列狭缝分离出选定的激发波长。光栅酶标仪可以截取光源波长范围内的任意波长，灵活性更高。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">&nbsp;&nbsp;&nbsp;&nbsp;<font face="宋体">目前，滤波系统由于其价格相对便宜、检测灵敏度高、带宽选择性强、滤波器切换速度快等特点，得到了较为广泛的应用。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">&nbsp;&nbsp;&nbsp;&nbsp;<strong><font face="宋体">微孔板读数仪选择</font></strong></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">&nbsp;&nbsp;&nbsp;&nbsp;<font face="宋体">现在您了解了原理，那么如何选择您喜欢的微孔板读数仪呢？可以考虑以下参数：</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">&nbsp;&nbsp;&nbsp;&nbsp;<font face="Calibri">1.</font><font face="宋体">测量波长</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">&nbsp;&nbsp;&nbsp;&nbsp;<font face="宋体">一般酶标仪的测量波长在</font><font face="Calibri">400~750nm</font><font face="宋体">之间或</font><font face="Calibri">800nm</font><font face="宋体">，完全可以满足</font><font face="Calibri">ELISA</font><font face="宋体">的显色测定。目前常见的</font><font face="Calibri">ELISA</font><font face="宋体">试剂盒所用的标记酶主要为辣根过氧化物酶（</font><font face="Calibri">HRP</font><font face="宋体">），底物通常为四甲基联苯胺（</font><font face="Calibri">TMB</font><font face="宋体">），在酸性或碱性终止液作用下，最终生成的黄色或蓝色产物的最大吸收波长分别在</font><font face="Calibri">450-490nm</font><font face="宋体">和</font><font face="Calibri">630-650nm</font><font face="宋体">左右，因此可根据实验需求选择仪器。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">&nbsp;&nbsp;&nbsp;&nbsp;<font face="Calibri">2.</font><font face="宋体">测量吸光度范围</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">&nbsp;&nbsp;&nbsp;&nbsp;<font face="宋体">通常酶标仪的吸光度测量范围在</font><font face="Calibri">0~2.5</font><font face="宋体">之间，可以满足</font><font face="Calibri">ELISA</font><font face="宋体">的测定要求。早期酶标仪能够测的吸光度一般在</font><font face="Calibri">0~2.5</font><font face="宋体">之间，现在基本已经拓宽到</font><font face="Calibri">3.5</font><font face="宋体">以上，并且能够保持良好的精密度和线性。因此，对于酶标仪的吸光度范围，不必刻意追求大的吸光度范围，主要看在一定吸光度范围内的线性和精密度。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">&nbsp;&nbsp;&nbsp;&nbsp;<font face="Calibri">3.</font><font face="宋体">检测速度</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">&nbsp;&nbsp;&nbsp;&nbsp;<font face="宋体">酶标仪的检测速度是指完成一次比色测定所需的时间。酶标仪的检测速度快，有助于提高检测的精度，即避免测定过程中因测定时间不同而导致每个微孔之间吸光度的差异。目前市面上常见的酶标仪的检测速度很快，通常在几秒钟之内。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">&nbsp;&nbsp;&nbsp;&nbsp;<font face="Calibri">4.</font><font face="宋体">振动盘功能</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">&nbsp;&nbsp;&nbsp;&nbsp;<font face="宋体">酶标仪的摇板功能是指酶标仪在进行比色测定前，对酶标板的孔板进行震荡混匀，使孔内颜色均匀一致。目前市面上常见的酶标仪都具备摇板功能，区别在于摇板的方式，有的可以通过上下、左右或旋转，以及振荡幅度任意调节。使用带有摇板功能的酶标仪进行</font><font face="Calibri">ELISA</font><font face="宋体">测定，显色反应完成后加入终止液，无需震荡混匀，直接放入酶标仪进行测定即可。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">&nbsp;&nbsp;&nbsp;&nbsp;<font face="Calibri">5.</font><font face="宋体">软件功能</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">&nbsp;&nbsp;&nbsp;&nbsp;<font face="宋体">软件功能是指酶标仪对</font><font face="Calibri">ELISA</font><font face="宋体">定性、定量测定以及其他测定方法（如酶标动力学、紫外、凝集等）的结果进行统计分析和报告的功能。软件功能是中高端酶标仪非常重要的功能。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">&nbsp;&nbsp;&nbsp;&nbsp;<font face="宋体">软件可以在读数前设置读数孔、波长、计算公式等，并在读数后显示相关数据。对于用户来说，良好的软件功能将对实际工作有很大的帮助。在</font><font face="Calibri">ELISA</font><font face="宋体">定性测定中，如果酶标仪存在阳性判断值（</font><font face="Calibri">cut-off</font><font face="宋体">），而其测定结果存在&ldquo;灰色区域&rdquo;（即测得的吸光度在</font><font face="Calibri">cut-off</font><font face="宋体">周围的一定区域内，则该区域内的结果应为&ldquo;可疑&rdquo;），统计计算功能不仅方便了实验室工作人员，在某些特定情况下也具有很高的实用价值。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">&nbsp;&nbsp;&nbsp;&nbsp;<font face="宋体">回到我们最初的问题：如何避免因酶标仪的失误而让数小时的心血付诸东流？答案就在于正确的认知和选择。希望通过今天的介绍，您对酶标仪的原理和挑选方法有了更清晰的认识。选择一台对的设备，不仅是投资，更是为您的珍贵样品和宝贵时间上一道保险。未来，更加智能、可靠的酶标仪必将成为您实验室中值得信赖的伙伴。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>