自身抗体-抗细胞因子抗体

<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">1<font face="宋体">．致病效果 抗细胞因子抗体的致病性没有清楚，可是上述自身抗体的</font><font face="Calibri">Fab</font><font face="宋体">片段具有特异性低、高亲和力结合相应细胞因子的才能。实际上，在正常人血清中抗</font><font face="Calibri">IL-la</font><font face="宋体">，</font><font face="Calibri">IL-6</font><font face="宋体">的自身抗体是其惟一且重要的结合蛋白。虽然现在对血清中的</font><font face="Calibri">IgG</font><font face="宋体">可以进行分类检测，但</font><font face="Calibri">IFN</font><font face="宋体">&alpha;、</font><font face="Calibri">IL10</font><font face="宋体">的自身抗体却无法检测到。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">或许的原因是自身抗体与其相应的细胞因子以免疫复合物的构成存在于血清中，或者是血清中存在某种克制因子。欲将复合物中的抗原、抗体分开，因抗体的高亲和力而不易抵达意图。</span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">至今仍未理解针对这些细胞因子的高亲和力的自身抗体为什么发生、是怎样发生的。这些自身抗体在体外能中和细胞因子，是否在体内也是这样，是否他们具有载体或保护细胞因子的功用等等都没有明了。</span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋体">可是，以细胞因子</font>-IgG<font face="宋体">复合物的方法使循环中的细胞因子得以安稳存在，这一点值得是肯定的。由此也从药动力学上解说了用抗</font><font face="Calibri">IL-6</font><font face="宋体">抗体治疗的患者中为什么出现细胞因子累积，原因是细胞因子</font><font face="Calibri">-IgG</font><font face="宋体">复合物较长的半衰期。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋体">可是，研讨也发现抗细胞因子特异抗体的</font>Fab<font face="宋体">段可以中和性结合上述细胞因子，其结合十分特异、高亲和力。细胞因子中和抗体的出现或许会中和其生物活性，而导致细胞因子治疗的失利。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">IL-l<font face="宋体">&alpha;是活化</font><font face="Calibri">T</font><font face="宋体">细胞的一种重要协同刺激因子，一同也或许是</font><font face="Calibri">Th2</font><font face="宋体">细胞的一种自分泌生长因子，因而，作为天然的免疫调节因子，抗</font><font face="Calibri">IL-l</font><font face="宋体">&alpha;抗体特别有意义。通过结合可溶性和膜相关</font><font face="Calibri">IL-la</font><font face="宋体">，克制细胞因子两种方法的活性，抗</font><font face="Calibri">IL-l</font><font face="宋体">&alpha;自身抗体势必会影响</font><font face="Calibri">IL1</font><font face="宋体">&alpha;的功用。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">2<font face="宋体">．检测方法 检测抗细胞因子抗体的方法有两大类。即生物学方法和免疫学方法。免疫学方法包含：</font><font face="Calibri">ELISA</font><font face="宋体">、放射免疫分析（</font><font face="Calibri">RIA</font><font face="宋体">）、免疫放射测定法、蛋白质印迹法（免疫印迹法）；</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">生物学方法主要是检测细胞因子的各种生物学功用，如克制实验、抗</span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">b<font face="宋体">毒</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋体">中和实验（抗</font>-IFN<font face="宋体">抗体）、</font><font face="Calibri">ILq</font><font face="宋体">抗体的中性粒细胞吸附克制实验以及胸腺细胞增殖克制实验。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">3<font face="宋体">．意义抗</font><font face="Calibri">IL-l</font><font face="宋体">&alpha;抗体含量在免疫性疾病中变化相当大；类风湿关节炎和全身性硬化症患者中抗</font><font face="Calibri">IL-6</font><font face="宋体">的高亲和力自身抗体水平增高。与之相反，抗</font><font face="Calibri">IL-l</font><font face="宋体">&alpha;抗体不出现在一般免疫炎性疾病，包含克隆病。酒精性肝硬化患者出现这些自身抗体意味着预后不良。测方法学上的缺乏阻止了细胞因子自身抗体意义的研讨。低水平细胞因子自身抗体对疾病发展无关紧要，或许仅仅反映在活动性炎症中相关细胞因子组成添加，随同自身抗体的增高</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">&nbsp;</span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>