酶联生物为您解析elisa检测结果不理想的原因

<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">e</span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">lisa<font face="宋体">作为经典的免疫检测方法，看起来很平常，然而真正做好它并不容易。有哪些方法可以借鉴呢？我们总结了几点：</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="color: rgb(255, 0, 0);"><strong><span style="font-family: 宋体; font-size: 9pt;">1.</span></strong></span><span style="background-color: rgb(255, 204, 153);"><span style="color: rgb(255, 0, 0);"><strong><span style="font-family: 宋体; font-size: 9pt;">&nbsp;</span></strong></span><strong><span style="font-family: 宋体; font-size: 9pt;">确定您的样本类型和试剂盒可以兼容</span></strong></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋体">常见样本类型有血清，血浆，细胞培养上清，如果是厂家实验验证过的，可根据产品说明购买使用。【注意：血浆制备用到的抗凝剂有</font> EDTA<font face="宋体">、柠檬酸盐、肝素等多种，相应的血浆性质存在差异，需单独确认兼容性】。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="color: rgb(255, 0, 0);"><strong><span style="font-family: 宋体; font-size: 9pt;">2.</span></strong></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">&nbsp;</span><span style="background-color: rgb(255, 204, 153);"><strong><span style="font-family: 宋体; font-size: 9pt;">试剂盒检测范围需要匹配样本中标志物浓度</span></strong></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><strong><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋体">elisa试剂盒</font></span></strong><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋体">的定量检测范围需参考标准曲线，在标准曲线最低和最高浓度区间内属于线性范围，其中的值是可信和可定量的。浓度高于线性范围的样品要稀释到线性范围内来测量，而浓度低于线性范围的样品，其测量值不能用于计算浓度，只能用做参考。有一种常见的相关情况是：健康人样本中很多标志物的浓度偏低，测量值低于标准曲线最低值。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="color: rgb(255, 0, 0);"><strong><span style="font-family: 宋体; font-size: 9pt;">3.</span></strong></span><strong><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">&nbsp;</span><span style="background-color: rgb(255, 204, 153);"><span style="font-family: 宋体; font-size: 9pt;">样本收集有讲究</span></span></strong><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋体">以血清为例，样本收集可参考试剂盒说明书，但需注意以下要点。样本尽量用无菌管收集，避免细菌的酶类和代谢产品对结果的影响。采集过程要避免溶血，因为红细胞溶解释放的活性物质可能会影响检测。保存过程中如有沉淀，应离心后取上清液。样本若不立即测定，建议按照每次使用量分装保存在</font> -20<font face="宋体">℃，每次检测使用一管，即避免交叉污染和反复冻融，有能保证检测结果的平行可比性。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="color: rgb(255, 0, 0);"><strong><span style="font-family: 宋体; font-size: 9pt;">4.</span></strong></span><strong><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">&nbsp;</span><span style="background-color: rgb(255, 204, 153);"><span style="font-family: 宋体; font-size: 9pt;">实验前要准备好相应试剂</span></span></strong><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋体">提前将所有试剂平衡到室温环境，这个过程大致需半小时左右。洗液是浓缩液，如有结晶析出，需完全溶解后再进行稀释。</font>A<font face="宋体">、</font><font face="Calibri">B </font><font face="宋体">两管显色底物在使用前</font><font face="Calibri">15</font><font face="宋体">分钟按 </font><font face="Calibri">1:1 </font><font face="宋体">配成混合液。为保证蛋白标准品溶液配制质量，蛋白标准品为冻干粉，重溶前需将管子离心，加入说明书指定的缓冲液，慢速在摇床上摇匀或颠倒混匀，至少 </font><font face="Calibri">15 </font><font face="宋体">分钟，不建议用枪头吹打。溶解后如需保存，按每次使用量分装后根据说明书要求的温度保存。梯度稀释蛋白标准品时，建议用聚丙烯管（对蛋白吸附力很低），每步都要充分混匀且更换新枪头，确保梯度准确性。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="color: rgb(255, 0, 0);"><strong><span style="font-family: 宋体; font-size: 9pt;">5.</span></strong></span><strong><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">&nbsp;</span><span style="background-color: rgb(255, 204, 153);"><span style="font-family: 宋体; font-size: 9pt;">仪器设备的准备也必不可少</span></span></strong><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋体">相关设备包括移液器、洗板机</font>/<font face="宋体">摇床和酶标仪。移液器的准确性对于</font><font face="Calibri">ELISA</font><font face="宋体">实验结果的可靠性十分关键，需要确保定期校准维护。摇床的使用是为了让反应体系快速达到平衡，获得稳定、可重复的结果。不用摇床对实验通常的影响是，</font><font face="Calibri">OD </font><font face="宋体">值低，</font><font face="Calibri">CV </font><font face="宋体">大。酶标仪等设备使用前提前 </font><font face="Calibri">15 </font><font face="宋体">分钟开机预热。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="color: rgb(255, 0, 0);"><strong><span style="font-family: 宋体; font-size: 9pt;">6.</span></strong></span><strong><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">&nbsp;</span><span style="background-color: rgb(255, 204, 153);"><span style="font-family: 宋体; font-size: 9pt;">剩余的试剂盒材料要妥善保存</span></span></strong><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋体">标准品分装后按说明书要求的温度保存，这样可以避免污染，对要求冷冻保存的标准品还可以避免反复冻融；酶标板放回锡箔纸袋，加入干燥剂，封紧封口，</font>4<font face="宋体">℃保存。忌未将酶标板完全平衡至室温，就放回锡箔纸袋，因为此时由于温度差，酶标板上会有水汽，不利于稳定保存。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="color: rgb(255, 0, 0);"><strong><span style="font-family: 宋体; font-size: 9pt;">7.</span><span style="font-family: 宋体; font-size: 9pt;">&nbsp;</span></strong></span><span style="background-color: rgb(255, 204, 153);"><strong><span style="font-family: 宋体; font-size: 9pt;">预实验：通常预实验包括全部标准曲线和小量样本。</span></strong></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">预实验有两个主要目的，一来是熟悉实验流程，发现可能忽略的问题；二来是测试样本和试剂盒是否匹配，一旦出现不匹配的情况，不至于浪费过多样本。另外是对样品中目的分析物的丰度作一下大致了解，以确定合适稀释度。</span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="color: rgb(255, 0, 0);"><strong><span style="font-family: 宋体; font-size: 9pt;">8.</span><span style="font-family: 宋体; font-size: 9pt;">&nbsp;</span></strong></span><span style="background-color: rgb(255, 204, 153);"><strong><span style="font-family: 宋体; font-size: 9pt;">加样要快速准确，避免气泡</span></strong></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋体">加样时间宜控制在</font> 15 <font face="宋体">分钟内。加样前要将样本混匀，特别是冻存后融化的样本（自然融化的血清等样本会有分层现象），应上下颠倒充分混匀，注意动作轻缓，避免产生气泡。如冻存融化后的样本有沉淀，可以再次离心。整个加样过程都要注意避免产生气泡。气泡会影响蛋白的相互结合，而影响反应进行。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="color: rgb(255, 0, 0);"><strong><span style="font-family: 宋体; font-size: 9pt;">9.</span><span style="font-family: 宋体; font-size: 9pt;">&nbsp;</span></strong></span><strong><span style="background-color: rgb(255, 204, 153);"><span style="font-family: 宋体; font-size: 9pt;">孵育时要保证温度均匀，防止挥发变干</span></span></strong><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">孵育时压紧封口膜。多块板一起实验时，要平放各板，不要叠放，以免因温度不均造成漂移或边缘效应。</span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="color: rgb(255, 0, 0);"><strong><span style="font-family: 宋体; font-size: 9pt;">1</span><span style="font-family: 宋体; font-size: 9pt;">0</span><span style="font-family: 宋体; font-size: 9pt;">.</span><span style="font-family: 宋体; font-size: 9pt;">&nbsp;</span></strong></span><strong><span style="background-color: rgb(255, 204, 153);"><span style="font-family: 宋体; font-size: 9pt;">手动洗板的操作指南</span></span></strong><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋体">加洗液要快速，没有多道移液器可以使用洗瓶。洗液应新鲜配制，以免被其他试剂污染，或染菌。加好洗液后浸泡</font> 30s-1min<font face="宋体">。甩板倒液要迅速干脆。倒液后在吸水纸上拍板，选用无粉尘和碎屑的优质吸水纸。需要用力拍板，使孔内没有可见液体挂壁；但也不能太重，不能让样品干太久。酶标板拍干后立即做后续的加液。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="color: rgb(255, 0, 0);"><strong><span style="font-family: 宋体; font-size: 9pt;">1</span><span style="font-family: 宋体; font-size: 9pt;">1</span><span style="font-family: 宋体; font-size: 9pt;">.</span></strong></span><strong><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">&nbsp;</span><span style="background-color: rgb(255, 204, 153);"><span style="font-family: 宋体; font-size: 9pt;">显色反应的关键点</span></span></strong><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><strong><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋体">e</font></span></strong><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋体"><strong>lisa试剂盒</strong>实验是个酶促底物显色反应，随时间增加，显色变深，所以选择最佳时间终止反应是得到准确的标准曲线和样本浓度的重要因素。终止过程要完全。使用的 </font><font face="Calibri">TMB </font><font face="宋体">底物时完全终止点是黄色，不会有任何程度的蓝色或绿色，终止过程中必要时可以震荡 </font><font face="Calibri">96 </font><font face="宋体">孔板，或用枪头抽吸混匀（终止液含强酸，避免腐蚀）。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="background-color: rgb(255, 255, 0);"><span style="font-family: 宋体; font-size: 9pt;">掌握了这些技巧与经验，你离实验成功只剩一个盒子的距离，酶联生物作为生物制剂厂家，无论从质量、价格还是售后上都有非常大的优势，赶紧去我们网站挑选符合实验课题的产品吧，为您的科研之路负责！</span></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>