ELISA产品选择指南
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">ELISA<font face="宋体">方法从</font><font face="Calibri">1970</font><font face="宋体">年代发展到现在,已经是一项实验室基本的免疫检测技术,广泛的应用于基础研究。</font></span></p>
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<p class="MsoNormal"><span style="color: rgb(255, 0, 0);"><strong><span style="font-family: 宋体; font-size: 9pt;">1. <font face="宋体">试剂盒特异性</font></span></strong></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">ELISA<font face="宋体">试剂盒的特异性与试剂盒的关键组分</font><font face="Calibri">--</font><font face="宋体">抗体对有关,若抗体对中之一为多抗,另一个必须为单抗,建议使用双单抗。在选择市面上的试剂盒时,最好先阅读相关产品的说明书,了解试剂盒的特异性和交叉反应性。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
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<p class="MsoNormal"><span style="color: rgb(255, 0, 0);"><strong><span style="font-family: 宋体; font-size: 9pt;">2.<font face="宋体">灵敏度和检测范围</font><font face="Calibri"> </font></span></strong></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋体">灵敏度反映的是试剂盒检出被检物质的最低限,用户可根据自己样本中待检指标的量选择合适的试剂盒,如果待检指标水平很低,一般的试剂盒不能满足要求,可选择高敏的</font>ELISA<font face="宋体">试剂盒。如果待检指标的浓度高于试剂盒标准曲线的最高值,则需要适当倍数稀释样本,计算结果时,乘以相应的系数倍数即可,如果样本中待检指标含量较低,一般不建议再做稀释。</font><font face="Calibri"> </font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
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<p class="MsoNormal"><span style="color: rgb(255, 0, 0);"><strong><span style="font-family: 宋体; font-size: 9pt;">3. <font face="宋体">重复性</font></span></strong></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋体">科学实验讲求重复性,一般</font>ELISA<font face="宋体">试剂盒,其板内和板间变异系数应该控制在</font><font face="Calibri">15%</font><font face="宋体">以内。在选择试剂盒时,要通过阅读相应的说明书或电话咨询厂家试剂盒的相关技术参数。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
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<p class="MsoNormal"><span style="color: rgb(255, 0, 0);"><strong><span style="font-family: 宋体; font-size: 9pt;">4. <font face="宋体">经济性</font><font face="Calibri"> </font></span></strong></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋体">对于选择进口品牌的试剂盒还是国产品牌的试剂盒,可根据实验室课题经费以及各品牌的产品质量和美誉度来自行选择。国际知名品牌一般质量可靠,文献引用多。建议大家通过阅读</font>SCI<font face="宋体">文献,了解同行所使用的品牌。但国际品牌由于进口和国际运输等成本较高,因此售价也较高。国产品牌,</font><font face="Calibri"> </font><font face="宋体">因省去不少物流和报关费用,与国外进口试剂盒相比价格上有比较大的优惠,而且能提供比较灵活的包装规格</font><font face="Calibri">, </font><font face="宋体">如</font><font face="Calibri">48tests</font><font face="宋体">小包装。</font><font face="Calibri"> </font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
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<p class="MsoNormal"><span style="color: rgb(255, 0, 0);"><strong><span style="font-family: 宋体; font-size: 9pt;">5. <font face="宋体">美誉度</font></span></strong></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋体">不论选择国际品牌还是国产品牌,都要注意产品的质量、知名度和美誉度。不是说所有的国外品牌质量都可靠,有些国际品牌其实是</font>OEM<font face="宋体">贴牌的,质量不一定有保障。还有一些公司提供的试剂盒,就更不靠谱了,相关指标的抗体,找遍全球都没有,如何开发出相应的</font><font face="Calibri">ELISA</font><font face="宋体">试剂盒呢?</font><font face="Calibri"> </font><font face="宋体">所以对于新手而言,一定要谨慎选择。比较可靠的国际品牌如美国的</font><font face="Calibri">R&D systems(</font><font face="宋体">细胞因子等</font><font face="Calibri">), eBioscience </font><font face="宋体">(细胞因子等)</font><font face="Calibri">(</font><font face="宋体">现被</font><font face="Calibri">Affymetrxi</font><font face="宋体">收购</font><font face="Calibri">), Caymanchem(</font><font face="宋体">主要为竞争法</font><font face="Calibri">EIA</font><font face="宋体">检测小分子物质</font><font face="Calibri">)。</font></span></p>