ELISA试剂盒中几大要点影响非特异性显色的

<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋体">影响</font><strong>ELISA<font face="宋体">试剂盒</font></strong><font face="宋体">中非特异性显色的缘由许多，如试剂盒特异性、查验标本中含酶符号物的烦扰物，操作进程中的疑问。这篇文章就这一缘由作一简明剖析</font><font face="Calibri">,</font><font face="宋体">以便能够经过以上办法把非特异显色降至最低极限，然后行进检测的特异性，并得到更精确、牢靠的试验作用。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">查验标本中富含酶符号物的烦扰物</span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋体">内源性烦扰物：类风湿因子</font>(RF)<font face="宋体">、黄疸等，类风湿因子是可作用于多种动物以及人</font><font face="Calibri">IgGFc</font><font face="宋体">段的本身抗体，大都为</font><font face="Calibri">IgM</font><font face="宋体">类，能充任抗原成分与固相及酶标抗体反响，然后出现非特异性显色。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">黄疸血标本中常富含内源性过氧化物酶，如用辣根过氧化物酶为符号物，就有能够发作非特异性显色。</span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋体">外源性烦扰物，常常因样品收集、储存、处置不妥构成如样品溶血，被细菌污染，标本凝聚不全等。溶血标本，红细胞溶解割裂，释放出血红素构成，而血红素中的铁卟啉是过氧化物酶的类似物，以</font>HRP<font face="宋体">为符号的</font><font face="Calibri">ELISA</font><font face="宋体">测定中，溶血标本能够会添加非特异性显色。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋体">如有细菌污染，菌体中能够富含内源性</font>HRP<font face="宋体">，有国内报导酶免</font><font face="Calibri">HAg</font><font face="宋体">试剂检测溶血标本时可构成假阳性。如在冰箱中保管过久，其间的</font><font face="Calibri">IgG</font><font face="宋体">可发作聚合，在直接法</font><font face="Calibri">ELISA</font><font face="宋体">试剂盒中可使本底加深。因而，血标本在收集处置时应留心，在冰箱中保管不易过久。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>