elisa试剂盒的检测前准备工作及注意事项

<p class="MsoNormal"><strong><span style="color: rgb(255, 0, 0);"><span style="font-family: 宋体; font-size: 9pt;">检测前准备工作：</span></span></strong><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><strong><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">1.</span></strong><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"> <font face="宋体">请提前</font><font face="Calibri">20</font><font face="宋体">分钟从冰箱中取出试剂盒，以平衡至室温。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><strong><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">2. </span></strong><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋体">将浓缩洗涤液用双蒸水稀释（</font><font face="Calibri">1:20</font><font face="宋体">）。未用完的放回</font><font face="Calibri">4</font><font face="宋体">℃。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><strong><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">3.</span></strong><span style="font-size: 9pt;"> <font face="宋体" style="font-family: 宋体;">标准品</font></span><span style="font-family: 宋体;">：</span><span style="font-size: 9pt;"><font face="宋体" style="font-family: 宋体;">加入标准品</font><font face="Calibri" style="font-family: 宋体;">/</font><font face="宋体" style="font-family: 宋体;">标本稀释液</font><font face="Calibri" style="font-family: 宋体;">0.5ml</font><font face="宋体" style="font-family: 宋体;">至冻干标准品中，静置</font><font face="Calibri" style="font-family: 宋体;">15</font><font face="宋体" style="font-family: 宋体;">分钟，待其充分溶解后，轻轻混匀（浓度为</font><font face="Calibri" style="font-family: 宋体;">1000 pg/ml</font><font face="宋体" style="font-family: 宋体;">）。然后根据需要进行稀释。（建议标准曲线使用以下浓度： </font><font face="Calibri" style="font-family: 宋体;">1000</font><font face="宋体" style="font-family: 宋体;">、</font><font face="Calibri" style="font-family: 宋体;">500</font><font face="宋体" style="font-family: 宋体;">、</font><font face="Calibri" style="font-family: 宋体;">250</font><font face="宋体" style="font-family: 宋体;">、</font><font face="Calibri" style="font-family: 宋体;">125</font><font face="宋体" style="font-family: 宋体;">、</font><font face="Calibri" style="font-family: 宋体;">62.5</font><font face="宋体" style="font-family: 宋体;">、</font><font face="Calibri" style="font-family: 宋体;">31.25</font><font face="宋体" style="font-family: 宋体;">、</font><font face="Calibri" style="font-family: 宋体;">15.6</font><font face="宋体" style="font-family: 宋体;">、</font><font face="Calibri" style="font-family: 宋体;">0 pg/ml</font><font face="宋体" style="font-family: 宋体;">）。复溶标准品原液（</font><font face="Calibri" style="font-family: 宋体;">1000 pg/ml</font><font face="宋体" style="font-family: 宋体;">）若未用完请分装后放入</font><font face="Calibri" style="font-family: 宋体;">-20</font><font face="宋体" style="font-family: 宋体;">℃以下电冰箱内保存，保存两个月。已稀释的标准品请废弃。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><strong><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">4.</span></strong><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"> <font face="宋体">生物素化抗体工作液：按当次试验所需要得用量，使用前</font><font face="Calibri">20</font><font face="宋体">分钟，以生物素化抗体稀释液稀释浓缩生物素化抗体（</font><font face="Calibri">1:30</font><font face="宋体">）成工作浓度。当日使用。若实验次数过多导致生物素化 抗体量不足，可向我们公司单独购买生物素化抗体。（须提供抗体批号）</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><strong><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">5.</span></strong><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"> <font face="宋体">酶结合物工作液：按当次试验所需要得用量，使用前</font><font face="Calibri">20</font><font face="宋体">分钟，以酶结合物稀释液稀释浓 缩酶结合物（</font><font face="Calibri">1:30</font><font face="宋体">） 成工作浓度。当日使用。若实验次数过多导致浓缩酶结合物量不足， 可向我们公司单独购买浓缩酶结合物。（须提供酶结合物批号）</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="color: rgb(255, 0, 0);"><strong><span style="font-family: 宋体; font-size: 9pt;">洗涤方法：</span></strong></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">1. <font face="宋体">自动洗板机：要求注入的洗涤液为</font><font face="Calibri">350ul</font><font face="宋体">，注入与吸出间隔</font><font face="Calibri">15</font><font face="宋体">－</font><font face="Calibri">30</font><font face="宋体">秒。洗板</font><font face="Calibri">5</font><font face="宋体">次。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">2. <font face="宋体">手工洗板：甩尽孔内液体，在洁净的吸水纸上拍干，每孔加洗涤液</font><font face="Calibri">350ul</font><font face="宋体">，静置</font><font face="Calibri">30</font><font face="宋体">秒后甩尽液体，在厚迭吸水纸上拍干。洗板</font><font face="Calibri">5</font><font face="宋体">次。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="color: rgb(255, 0, 0);"><strong><span style="font-family: 宋体; font-size: 9pt;">注意事项：</span></strong></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">1. <font face="宋体">试剂盒使用前请保存在</font><font face="Calibri">2-8</font><font face="宋体">℃。复溶后的标准品应分装后，将其放在</font><font face="Calibri">-20</font><font face="宋体">～</font><font face="Calibri">-70</font><font face="宋体">℃贮存。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">2. <font face="宋体">浓缩生物素化抗体，浓缩酶结合物体积小，运输中颠簸和可能的倒置，会使液体沾到管壁 或瓶盖。因此使用前请用手甩几下或</font><font face="Calibri">1000</font><font face="宋体">转</font><font face="Calibri">/</font><font face="宋体">分离心</font><font face="Calibri">1</font><font face="宋体">分钟，以使附着管壁或瓶盖的液体沉积到管底。取用前，请用移液器小心吹打</font><font face="Calibri">4-5</font><font face="宋体">次使溶液混匀。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">3. <font face="宋体">从冰箱中取出的浓缩洗涤液可能有结晶</font><font face="Calibri">,</font><font face="宋体">属于正常现象，微加热至</font><font face="Calibri">40</font><font face="宋体">℃使结晶完全溶解后 再配制洗涤液。（加热温度不要超过</font><font face="Calibri">50</font><font face="宋体">℃）使用时洗涤液应为室温。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">4. <font face="宋体">若需要分次使用标准品应按照每一次用量分装，将其放在</font><font face="Calibri">-20</font><font face="宋体">～</font><font face="Calibri">-70</font><font face="宋体">℃贮存，避免反复冻 融。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">5. <font face="宋体">不同批号的试剂盒组份不能混用（洗涤液和反应终止液除外）。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">6. <font face="宋体">充分轻微混匀对反应结果尤为重要，使用微量振荡器（使用</font><font face="Calibri">*</font><font face="宋体">频率），如无微量振荡器，可在反应前手工轻轻敲击酶标板框混匀。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">7. <font face="宋体">在试验中标准品和样本检测时建议作双孔检测。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">8. <font face="宋体">试验中所用的试管和吸头均为一次性使用，严禁在不同的液体之间混用！否则将影响试 验结果。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">9. <font face="宋体">试验中请穿着试验服并带乳胶手套做好防护工作，特别是检测血液或者其他体液标本时， 请按国家生物试验室安全防护条列执行。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>