白介素类的elisa试剂盒实验条件

<p class="MsoNormal"><span style="background-color: rgb(0, 255, 255);"><strong><span style="font-family: 宋体; font-size: 9pt;">实验条件</span></strong></span><span style="font-family: 宋体; font-size: 9pt;"><o:p></o:p></span></p> <p class="MsoNormal"><strong><span style="font-family: 宋体; font-size: 9pt;">elisa<font face="宋体">试剂盒</font></span></strong><span style="font-family: 宋体; font-size: 9pt;"><font face="宋体">白介素类检测抗体多用间接型</font><font face="Calibri">elisa</font><font face="宋体">，以纯化抗原包被载体，经</font><font face="Calibri">4</font><font face="宋体">℃过夜，以无关蛋白封闭至少</font><font face="Calibri">2</font><font face="宋体">小时以上，加入待测样品，室温孵育</font><font face="Calibri">1</font><font face="宋体">小时，洗涤干净后加酶标抗体，再经室温孵育、洗涤后，以底物显色，显色到一定程度终止反应，在酶标仪上读结果或以肉眼判断。</font></span><span style="font-family: 宋体; font-size: 9pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="color: rgb(255, 102, 0);"><strong><span style="font-family: 宋体; font-size: 9pt;">固相载体：</span></strong></span><span style="font-family: 宋体; font-size: 9pt;"><o:p></o:p></span></p> <p><font face="宋体" style="font-family: 宋体; font-size: 9pt;">可作为固相载体的物质很多，如：纤维素、聚丙烯酰胺、聚苯乙烯、聚丙烯、交联葡聚糖、玻璃、硅橡胶、琼脂糖凝胶等。可以各种形式出现；试管、微量反应板凹孔、小珠</font><span style="font-family: 宋体; font-size: 9pt;">.....</span><font face="宋体" style="font-family: 宋体; font-size: 9pt;">常用的是聚苯乙烯制品，其吸附性</font><font face="宋体" style="font-family: 宋体; font-size: 9pt;">好，固相包被的机制一般不外乎物理吸附和共价交联，</font><font face="宋体" style="font-family: 宋体; font-size: 9pt;">类</font><span style="font-family: 宋体; font-size: 9pt;"> &nbsp;</span><font face="宋体" style="font-family: 宋体; font-size: 9pt;">型：</font></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">elisa<font face="宋体">具体方法的类型繁多，试剂的标准化问题还未</font><font face="Calibri">zui</font><font face="宋体">后解决，温育的时间、温度、洗涤液的种类及洗涤次数等都是影响测定的因素。但参考以上基本过程，</font></span><strong><span style="font-family: 宋体; font-size: 9pt;">elisa<font face="宋体">试剂盒</font></span></strong><font face="宋体" style="font-family: 宋体; font-size: 9pt;">无论怎样变化，均离不开抗原</font><font face="Calibri" style="font-size: 9pt;">-</font><font face="宋体" style="font-family: 宋体; font-size: 9pt;">抗体反应、酶标抗体的免疫学反应及酶催化作用。</font></p> <p class="MsoNormal"><span style="color: rgb(255, 102, 0);"><strong><span style="font-family: 宋体; font-size: 9pt;"><font face="宋体">特</font> &nbsp;<font face="宋体">点：</font></span></strong></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">1<font face="宋体">、敏感性高，可检出浓度在</font><font face="Calibri">ug~mg/ml</font><font face="宋体">水平；</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">2<font face="宋体">、特异性强；</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">3<font face="宋体">、重复性好；</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">4<font face="宋体">、快速、简便、廉价。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="color: rgb(255, 102, 0);"><strong><span style="font-family: 宋体; font-size: 9pt;">封闭：</span></strong></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋体">以无关蛋白占据载体上未被包被的空隙，常用的封闭液是</font>1%<font face="宋体">牛血清白蛋白或</font><font face="Calibri">10%</font><font face="宋体">小牛血清。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="color: rgb(255, 102, 0);"><strong><span style="font-family: 宋体; font-size: 9pt;">待测样品：</span></strong></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋体">稀释度要合适，浓度太大会发生非特异性吸附造成假阳性，过分稀释会影响抗原</font>-<font face="宋体">抗体之间的免疫学反应。选择</font><font face="Calibri">zui</font><font face="宋体">适稀释度，在实验体系中取光密度在</font><font face="Calibri">1.0</font><font face="宋体">左右的稀释度为宜。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="color: rgb(255, 102, 0);"><strong><span style="font-family: 宋体; font-size: 9pt;">酶结合物：</span></strong></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><strong><span style="font-family: 宋体; font-size: 9pt;">elisa<font face="宋体">试剂盒</font></span></strong><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋体">使用高质量的酶结合物是</font>elisa<font face="宋体">成功的关键。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>