ELISA试剂盒常见问题

<div> <p class="MsoNormal"><span style="color: rgb(255, 0, 0);"><strong><span style="font-family: 宋体; font-size: 9pt;"><font face="宋体">这里就简单整理了</font>5<font face="宋体">个问题，具体内容如下：</font></span></strong></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">1<font face="宋体">、一个</font><strong><font face="Calibri">ELISA</font><font face="宋体">试剂盒</font></strong><font face="宋体">检测几个样品？</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">我司在各大论坛看到最多的三个答案：</span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">1<font face="宋体">）</font><font face="Calibri">48T </font><font face="宋体">可做</font><font face="Calibri">42</font><font face="宋体">个样本加</font><font face="Calibri">6</font><font face="宋体">个标准曲线；</font><font face="Calibri">96T </font><font face="宋体">可做</font><font face="Calibri">90</font><font face="宋体">个样本加</font><font face="Calibri">6</font><font face="宋体">个标准曲线</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">2<font face="宋体">）以</font><font face="Calibri">96T</font><font face="宋体">试剂盒来算，定量的试剂盒一般可以做</font><font face="Calibri">90</font><font face="宋体">个左右的样品，定性的试剂盒可以做</font><font face="Calibri">94</font><font face="宋体">个左右的样品</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">3<font face="宋体">）</font><font face="Calibri">96T</font><font face="宋体">的</font><font face="Calibri">ELISA</font><font face="宋体">试剂盒，去除标准品复孔检测，最多还能检测</font><font face="Calibri">80</font><font face="宋体">个标本；</font><font face="Calibri">48T</font><font face="宋体">的最多</font><font face="Calibri">30</font><font face="宋体">个样本。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">2<font face="宋体">、</font><font face="Calibri">elisa</font><font face="宋体">试剂盒</font><font face="Calibri">96T</font><font face="宋体">的能做检测多少血清样品？板能重复利用吗？</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">96T<font face="宋体">，一般做复孔的话，做标准曲线需要</font><font face="Calibri">16</font><font face="宋体">个孔，剩下</font><font face="Calibri">80</font><font face="宋体">个孔就可以做</font><font face="Calibri">40</font><font face="宋体">个样本。这个板不能重复利用。而且配套的试剂也只是够一块板子用的。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">3<font face="宋体">、</font><strong><font face="Calibri">ELISA</font><font face="宋体">试剂盒想</font></strong><font face="宋体">请教一下，我想用</font><font face="Calibri">elisa</font><font face="宋体">法测血清里的一种抗原，查了资料，发现没有现成的试剂盒用，现在就有</font><font face="Calibri">2</font><font face="宋体">种备选方案，一种是买抗体对自己包被（查了</font><font face="Calibri">R&amp;D</font><font face="宋体">等大公司都没有可用于</font><font face="Calibri">elisa</font><font face="宋体">的单抗）。哪种比较好呢？自己包被的会不会比国产的试剂盒可靠呢？</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">如果你觉得技术不错有队其他公司不放心，可以自己购买抗体对自己包被，这个就是麻烦点，自己包被用着放心，舒心；</span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">4<font face="宋体">、</font><font face="Calibri">western </font><font face="宋体">和</font><font face="Calibri">ELISA </font><font face="宋体">处理样本有何差别？</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">Western Blot <font face="宋体">样品制备要加蛋白裂解液（含有蛋白酶抑制剂），然后冰上研磨，收集于</font><font face="Calibri">EP</font><font face="宋体">管，冰上裂解</font><font face="Calibri">30min</font><font face="宋体">，</font><font face="Calibri">4</font><font face="宋体">度</font><font face="Calibri">12000rpm</font><font face="宋体">离心</font><font face="Calibri">15min</font><font face="宋体">，取上清分装，即得到蛋白样品。建议直接电泳，多余的</font><font face="Calibri">-80</font><font face="宋体">保存。</font><font face="Calibri">ELISA</font><font face="宋体">试剂盒建议分装的蛋白每支略多于一次上样量，一次性用完一支，避免反复冻融。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">ELISA<font face="宋体">样品，直接脑组织冰上匀浆，收集于</font><font face="Calibri">EP</font><font face="宋体">管，同上一样离心，大约</font><font face="Calibri">220-250ul</font><font face="宋体">每支分装（因为</font><font face="Calibri">ELISA</font><font face="宋体">上样量基本都是每孔</font><font face="Calibri">100ul</font><font face="宋体">，做复孔，每个样品</font><font face="Calibri">2</font><font face="宋体">孔，就需要</font><font face="Calibri">200ul</font><font face="宋体">，为了避免不够</font><font face="Calibri">2</font><font face="宋体">孔，分装时要大于</font><font face="Calibri">200ul</font><font face="宋体">每支），直接实验。多余的</font><font face="Calibri">-80</font><font face="宋体">保存，一样避免反复冻融。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋体">不过</font>ELISA<font face="宋体">最好少量样品进行预实验，或者查阅文献，看看大概组织里有多高浓度，选择合适的试剂盒，测量范围要与组织浓度相符。避免浓度过高测量不准，或者浓度过低，小于试剂盒最低可测浓度。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> </div>