荧光定量PCR技术服务

<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋体">荧光定量</font>PCR<font face="宋体">用</font><font face="Calibri">Taqman </font><font face="宋体">方法对 </font><font face="Calibri">DNA/RNA </font><font face="宋体">进行 </font><font face="Calibri">real time PCR </font><font face="宋体">的定量测定或型的鉴定。</font></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="color: rgb(255, 0, 0);"><strong><span style="font-family: 宋体; font-size: 9pt;"><font face="宋体">荧光定量</font>PCR<font face="宋体">操作程序</font><font face="Calibri">&nbsp;</font></span></strong></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><strong><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">①</span></strong><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"> 根据基因序列设计特异性的引物和荧光探针。<font face="Calibri">&nbsp;</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><strong><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">② </span></strong><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">提取 <font face="Calibri">DNA/ RNA </font><font face="宋体">。</font><font face="Calibri">&nbsp;</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><strong><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">③ </span></strong><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="Calibri">Real Time PCR(</font><font face="宋体">荧光定量</font><font face="Calibri">PCR)</font><font face="宋体">，进行实验结果分析</font><font face="Calibri">&nbsp;。</font><o:p></o:p></span></p> <p class="MsoNormal"><strong><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">④ </span></strong><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">实验完成后，提供完整的实验报告（含软件分析结果）及引物等实验材料<font face="Calibri">&nbsp;。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="color: rgb(255, 0, 0);"><strong><span style="font-family: 宋体; font-size: 9pt;"><font face="宋体">荧光定量</font>PCR<font face="宋体">收费标准</font><font face="Calibri">&nbsp;</font></span></strong></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">1<font face="宋体">、</font><font face="Calibri">RNA</font><font face="宋体">提取：</font><font face="Calibri">10</font><font face="宋体">－</font><font face="Calibri">20</font><font face="宋体">个样品的</font><font face="Calibri">RNA</font><font face="宋体">提取是：</font><font face="Calibri">150</font><font face="宋体">元</font><font face="Calibri">/</font><font face="宋体">个；</font><font face="Calibri">20</font><font face="宋体">－</font><font face="Calibri">30</font><font face="宋体">个样品的</font><font face="Calibri">RNA</font><font face="宋体">提取是：</font><font face="Calibri">120 </font><font face="宋体">元</font><font face="Calibri">/</font><font face="宋体">个；</font><font face="Calibri">30</font><font face="宋体">个以上样品的</font><font face="Calibri">RNA</font><font face="宋体">提取是：</font><font face="Calibri">100</font><font face="宋体">元</font><font face="Calibri">/</font><font face="宋体">个；每份材料</font><font face="Calibri">*</font><font face="宋体">少应提供：</font><font face="Calibri">100mg/</font><font face="宋体">样品。</font></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">2<font face="宋体">、一个指标每个样品为</font><font face="Calibri">150</font><font face="宋体">元；二个指标每个样品为</font><font face="Calibri">240</font><font face="宋体">元；三指标每个样品为</font><font face="Calibri">300</font><font face="宋体">元，三个指标以上每个样品为</font><font face="Calibri">80</font><font face="宋体">元</font><font face="Calibri">/</font><font face="宋体">反应。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">3<font face="宋体">、引物合成为每个碱基</font><font face="Calibri">2.1</font><font face="宋体">元，探针标记为</font><font face="Calibri">1200</font><font face="宋体">元</font><font face="Calibri">/</font><font face="宋体">条，如果是用</font><font face="Calibri">sybr green I(</font><font face="宋体">染料</font><font face="Calibri">)</font><font face="宋体">做，则免去探针的费用，染料赠送。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">4<font face="宋体">、内参的</font><font face="Calibri">GAPDH</font><font face="宋体">引物探针免费，标准曲线免费赠送。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">5<font face="宋体">、不再收取任何的基础费或者开机费。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="color: rgb(255, 0, 0);"><strong><span style="font-family: 宋体; font-size: 9pt;"><font face="宋体">荧光定量</font>PCR<font face="宋体">服务要求</font></span></strong></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="color: rgb(255, 102, 0);"><span style="font-family: 宋体; font-size: 9pt;">★</span></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"> 请您提供新鲜的且尽量多的材料（各种材料的 <font face="Calibri">RNA </font><font face="宋体">提取量请参照&ldquo;总 </font><font face="Calibri">RNA </font><font face="宋体">的提取&rdquo;），或直接提供纯化好的 总 </font><font face="Calibri">RNA </font><font face="宋体">（大于 </font><font face="Calibri">5 ug/ </font><font face="宋体">样品）；（ 各种材料的 </font><font face="Calibri">DNA </font><font face="宋体">提取量请参照&ldquo; </font><font face="Calibri">D NA </font><font face="宋体">的提取&rdquo;），或直接提供纯化好的 </font><font face="Calibri">D NA </font><font face="宋体">（大于 </font><font face="Calibri">5 ug/ </font><font face="宋体">样品 </font><font face="Calibri">) </font><font face="宋体">。</font><font face="Calibri">&nbsp;</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="color: rgb(255, 102, 0);"><span style="font-family: 宋体; font-size: 9pt;">★</span></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"> 请通过 <font face="Calibri">E-mail </font><font face="宋体">提供已知的全长基因序列。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="color: rgb(255, 102, 0);"><span style="font-family: 宋体; font-size: 9pt;">★ </span></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">请提供尽可能详细的背景资料： <font face="Calibri">DNA/ RNA </font><font face="宋体">来源、丰度等</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>