怎么区分ELISA试剂盒能否测手中的样本

<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋体">关于</font>zui<font face="宋体">常见的细胞上清，咱们都知道，培养细胞进程中，无分外状况一般是</font><font face="Calibri">10%</font><font face="宋体">的牛血清参与培养基中，经过培养后，细胞的吸收耗费。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋体">现在</font>ELISA<font face="宋体">试剂盒查看的样本中，除了细胞上清外，还有比较大的一部分是血清和血浆样本。关于</font><font face="Calibri">ELISA</font><font face="宋体">试剂盒客户来说，在试验中或许用到不一样的样本，怎么区分所购的</font><font face="Calibri">ELISA</font><font face="宋体">试剂盒能否测手中的样本呢？</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">zui<font face="宋体">后细胞上清中蛋白含量会很少，并且关于一般牛血清出产的厂家来说，在出产进程中现已去除了，所以对一般</font><font face="Calibri">ELISA</font><font face="宋体">而言，不会影响抗体抗原的。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋体">如</font>elisa<font face="宋体">试剂盒厂家没有供应专门用于血清血浆样本的缓冲液，那么只要用已知浓度的待测政策蛋白参与所测种属的血清作为样本加样和用已知浓度的待测政策蛋白参与</font><font face="Calibri">PBS</font><font face="宋体">缓冲液中一起查看对比，</font><font face="Calibri">ELISA</font><font face="宋体">试剂盒即可知道该试剂盒是不是能够直接用来测血清血浆样本。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">ELISA<font face="宋体">试剂盒灵敏度高是一大优点，而这其实也是和检测样本有些关联的，我们一般说来，如果待测样本中目标蛋白的浓度比较高，则对灵敏度要求相对小一些。如果待测样本中目标蛋白的浓度比较低，则必须考虑灵敏度的问题。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋体">一般说来，诊断试剂的检测范围以</font>ng/ml<font face="宋体">计。而常见的科研试剂盒中的样本中的目标蛋白，范围可随样本的类型而变化。所以实验前应通过文献和预实验的方法确定样本是否在所购的范围内，是很重要的步骤。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋体">一般来说，试剂盒曲线上的</font>zui<font face="宋体">小浓度是比较准确的，低于这个值因为与曲线是个&ldquo;</font><font face="Calibri">S</font><font face="宋体">&rdquo;型结构有关，所以结果是有偏差的，是一种估算。再加上实验误差，所以达不到理想的效果。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">建议选择试剂盒时，应该看曲线上小的浓度值，而不是试剂盒上写的灵敏度。</span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>