上海酶联为您详解白细胞介素2试剂盒的生物学活性测定

<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋体">上海酶联生物科技有限公司专业代理人白介素</font>elisa<font face="宋体">试剂盒，大鼠白介素</font><font face="Calibri">Elisa</font><font face="宋体">试剂盒，小鼠</font><font face="Calibri">Elisa</font><font face="宋体">试剂盒等多类种属，欢迎来电咨询第二季度优惠价格。我司提供免费技术指导及代测服务，为您的实验带来全方位的服务体验。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><strong><span style="color: rgb(255, 0, 0);"><span style="font-family: 宋体; font-size: 9pt;">★&nbsp;实验目的</span></span></strong><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><strong><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">①</span></strong><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"> </span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋体">掌握</font>IL-2<font face="宋体">生物学活性测定的原理。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><strong><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">②</span></strong><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"> </span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋体">熟悉</font>IL-2<font face="宋体">生物学活性测定的实验方法和结果计算。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><strong><span style="color: rgb(255, 0, 0);"><span style="font-family: 宋体; font-size: 9pt;">★</span><span style="font-family: 宋体; font-size: 9pt;">&nbsp;</span><span style="font-family: 宋体; font-size: 9pt;">实验原理</span></span></strong><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">IL-2<font face="宋体">是由</font><font face="Calibri">Th</font><font face="宋体">细胞产生的淋巴因子，在淋巴细胞增殖分化过程中起到非常重要的作用。</font><font face="Calibri">IL-2</font><font face="宋体">活性测定基于</font><font face="Calibri">IL-2</font><font face="宋体">能维持</font><font face="Calibri">IL-2</font><font face="宋体">依赖细胞的代谢和存活，促进这类细胞的增殖。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋体">细胞在增殖时能量代谢活跃，可产生大量的能量以供合成多种大分子物质和细胞分裂所需，能量代谢的水平与细胞合成</font>DNA<font face="宋体">水平基本平行，因此，测定细胞能量代谢的水平可以间接地反映细胞增殖情况。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">MTT<font face="宋体">（四甲基偶氮唑盐）是一种淡黄色的水溶性化合物，活细胞（特别是增殖期的细胞）通过线粒体能量代谢过程中的琥珀酸脱氢酶的作用，使淡黄色的</font><font face="Calibri">MTT</font><font face="宋体">分解产生蓝色结晶状甲臢沉积于细胞内或细胞周围，且形成甲臢的量与细胞增殖程度呈正比，甲臢经</font><font face="Calibri">SDS</font><font face="宋体">作用后可溶解显色。溶解液的光密度值与细胞代谢及</font><font face="Calibri">IL-2</font><font face="宋体">活性正相关。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><strong><span style="color: rgb(255, 0, 0);"><span style="font-family: 宋体; font-size: 9pt;">★</span><span style="font-family: 宋体; font-size: 9pt;">&nbsp;</span><span style="font-family: 宋体; font-size: 9pt;">实验材料</span></span></strong><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><strong><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">1.</span></strong><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">1640<font face="宋体">培养液（完全）、</font><font face="Calibri">IL-2</font><font face="宋体">标准品、待测</font><font face="Calibri">IL-2</font><font face="宋体">样品、</font><font face="Calibri">CTLL-2</font><font face="宋体">细胞株、</font><font face="Calibri">MTT</font><font face="宋体">溶液（</font><font face="Calibri">5mg/ml</font><font face="宋体">）、</font><font face="Calibri">10%SDS</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><strong><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">2.</span></strong><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">96<font face="宋体">孔细胞培养板、多头细胞收集器、微量加样器、</font><font face="Calibri">CO2</font><font face="宋体">孵箱、酶标仪</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><strong><span style="color: rgb(255, 0, 0);"><span style="font-family: 宋体; font-size: 9pt;">★</span><span style="font-family: 宋体; font-size: 9pt;">&nbsp;</span><span style="font-family: 宋体; font-size: 9pt;">实验方法</span></span></strong><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><strong><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">①</span></strong><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"> </span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋体">制备</font>CTLL-2<font face="宋体">细胞悬液</font><font face="Calibri">&nbsp; </font><font face="宋体">取生长旺盛的</font><font face="Calibri">CTLL-2</font><font face="宋体">细胞，用</font><font face="Calibri">1640</font><font face="宋体">培养液将细胞洗</font><font face="Calibri">3</font><font face="宋体">次，用完全</font><font face="Calibri">1640</font><font face="宋体">培养液配成</font><font face="Calibri">2</font><font face="宋体">&times;</font><font face="Calibri">105/ml</font><font face="宋体">细胞悬液。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><strong><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">②</span></strong><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"> </span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋体">稀释样品和标准品</font>&nbsp; <font face="宋体">将待测样品和标准</font><font face="Calibri">IL-2</font><font face="宋体">用完全</font><font face="Calibri">1640</font><font face="宋体">培养液做一定的倍比稀释。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><strong><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">③</span></strong><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"> </span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋体">加样与检测</font>&nbsp; <font face="宋体">向</font><font face="Calibri">96</font><font face="宋体">孔细胞培养板内加入不同稀释度的样品和标准品（</font><font face="Calibri">50</font><font face="宋体">&mu;</font><font face="Calibri">l/</font><font face="宋体">孔），每稀释度</font><font face="Calibri">3</font><font face="宋体">个复孔，并设细胞对照。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋体">再向各孔加入</font>50<font face="宋体">&mu;</font><font face="Calibri">l</font><font face="宋体">细胞悬液，混匀，置</font><font face="Calibri">5%CO237</font><font face="宋体">℃培养</font><font face="Calibri">36</font><font face="宋体">小时，每孔加入</font><font face="Calibri">MTT</font><font face="宋体">溶液</font><font face="Calibri">20</font><font face="宋体">&mu;</font><font face="Calibri">l CO2</font><font face="宋体">孵育</font><font face="Calibri">6</font><font face="宋体">～</font><font face="Calibri">8</font><font face="宋体">小时后，每孔再加</font><font face="Calibri">10%SDS100</font><font face="宋体">&mu;</font><font face="Calibri">l</font><font face="宋体">，充分混匀，</font><font face="Calibri">37</font><font face="宋体">℃孵箱静放（使甲臢全溶解）。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋体">在酶联仪上选波长</font>570nm<font face="宋体">测定</font><font face="Calibri">OD</font><font face="宋体">值，将待测样品的</font><font face="Calibri">OD</font><font face="宋体">值与标准品</font><font face="Calibri">OD</font><font face="宋体">值比较后，求得待测样品的</font><font face="Calibri">IL-2</font><font face="宋体">活性单位。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><strong><span style="color: rgb(255, 0, 0);"><span style="font-family: 宋体; font-size: 9pt;">★</span><span style="font-family: 宋体; font-size: 9pt;">&nbsp;</span><span style="font-family: 宋体; font-size: 9pt;">结果计算</span></span></strong><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋体">将各稀释度的</font>OD<font face="宋体">值按照样品增殖</font><font face="Calibri">OD</font><font face="宋体">值的百分比换算成概率单位，可将原来呈</font><font face="Calibri">S</font><font face="宋体">形的曲线转换成为直线。根据这些点的数据求出各直线的回归方程。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋体">再从回归方程求出各样品达</font>50%*<font face="宋体">增殖时的稀释度，而后按下列公式求出待测样品</font><font face="Calibri">IL-2</font><font face="宋体">的活性单位。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><strong><span style="color: rgb(255, 0, 0);"><span style="font-family: 宋体; font-size: 9pt;">★</span><span style="font-family: 宋体; font-size: 9pt;">&nbsp;</span><span style="font-family: 宋体; font-size: 9pt;">计算公式：</span></span></strong><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><strong><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">&nbsp;x&nbsp; </span></strong><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋体">待测样品</font><font face="Calibri">IL-2</font><font face="宋体">活性单位（&mu;</font><font face="Calibri">/ml</font><font face="宋体">）；</font><font face="Calibri">&nbsp; a</font><font face="宋体">：标准参考样品</font><font face="Calibri">IL-2</font><font face="宋体">的活性单位（&mu;</font><font face="Calibri">/ml</font><font face="宋体">）；</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><strong><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">&nbsp;d&nbsp; </span></strong><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋体">待测样品达</font><font face="Calibri">50%*</font><font face="宋体">增殖的稀释度；</font><font face="Calibri">&nbsp; D</font><font face="宋体">：标准参考样品在</font><font face="Calibri">50%*</font><font face="宋体">增殖的稀释度。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><strong><span style="color: rgb(255, 0, 0);"><span style="font-family: 宋体; font-size: 9pt;">★</span><span style="font-family: 宋体; font-size: 9pt;">&nbsp;</span><span style="font-family: 宋体; font-size: 9pt;">注意事项</span></span></strong><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><strong><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">1<font face="宋体">．</font><font face="Calibri">&nbsp; </font></span></strong><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="Calibri">MTT</font><font face="宋体">液要现配现用，避免光照，若有蓝色颗粒需过滤后再用。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><strong><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">2<font face="宋体">．</font></span></strong><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="Calibri">&nbsp; </font><font face="宋体">生物学测定法敏感性高，特异性强，所测</font><font face="Calibri">IL-2</font><font face="宋体">是具有生物活性的</font><font face="Calibri">IL-2</font><font face="宋体">，而不象免疫学测定法所测定免疫反应性</font><font face="Calibri">IL-2</font><font face="宋体">蛋白，因此测定的条件和要求要严格按规程。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>