依照ELISA试剂盒说明书请求用处

<p class="MsoNormal"><strong><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">ELISA<font face="宋体">试剂盒</font></span></strong><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋体">检查试剂每次实验、每块扳上都要设置以下</font><font face="Calibri">3</font><font face="宋体">项对照和用处：</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">1<font face="宋体">．空白对照：仅用稀释液替代检查样本、</font><font face="Calibri">ELISA</font><font face="宋体">试剂盒用来观察终究反响的显色&ldquo;本底</font><font face="Calibri">&quot;</font><font face="宋体">、</font><font face="Calibri">ELISA</font><font face="宋体">试剂盒并用于酶标仪消除、扣减&ldquo;本底</font><font face="Calibri">&quot;</font><font face="宋体">，也即是说：以本底为&ldquo;零</font><font face="Calibri">&quot;</font><font face="宋体">读取阳性、阴性对照孔和样本检查孔的吸光值（</font><font face="Calibri">A</font><font face="宋体">）；或许，在核算阴性对照均值（</font><font face="Calibri">NCx</font><font face="宋体">）、阳性对照均值（</font><font face="Calibri">PCx</font><font face="宋体">）、样本读数的</font><font face="Calibri">S/C.O.</font><font face="宋体">值时减去空白对照的本底吸光值。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋体">前期的酶标仪没有主动扣减空白对照孔读数的功用，</font>ELISA<font face="宋体">试剂盒这种酶标仪现在现已被筛选了，写上这段话的意图是弥补说明设置空白对照的用处。空白孔详细怎么读取读数，应当依照说明书操作。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">2<font face="宋体">．阴性对照：</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋体">（</font>1<font face="宋体">） </font><strong><font face="Calibri">ELISA</font><font face="宋体">试剂盒</font></strong><font face="宋体">阴性对照的意义与请求：</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">ELISA<font face="宋体">试剂盒所谓阴性对照，是本次检查实验中选用与待检查物（样品、人用试剂即是人的血清等）具有同源性和同质性，又不富含待检物质，而且可以客观比照和区分处理要素（血清免疫学实验中有特异性抗原与抗体反响）之间的区别。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋体">因而，用动物血清或其成品替代阴性人血清作为对照品，无论是从理论仍是实践上都是不可取的。留意：据我所知，国产</font>ELISA<font face="宋体">试剂盒中，有的厂家是用动物血清成品稀释装备或与有些人血清混合装备的；阴性对照读数，并不是&ldquo;越低越好</font><font face="Calibri">&quot;</font><font face="宋体">。</font><font face="Calibri">NCx</font><font face="宋体">值挨近</font><font face="Calibri">0.00X</font><font face="宋体">水平，这是假象！</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋体">试想一下，真实选用</font>&ldquo;阴性人血清<font face="Calibri">&quot;</font><font face="宋体">的阴性对照品，</font><font face="Calibri">NCx</font><font face="宋体">值会如此之低吗？例如，雅培乙肝六项</font><font face="Calibri">ELISA</font><font face="宋体">试剂盒的</font><font face="Calibri">NCx</font><font face="宋体">值，分别为：</font><font face="Calibri">0.010</font><font face="宋体">（</font><font face="Calibri">HBsAg</font><font face="宋体">）、</font><font face="Calibri">0.027</font><font face="宋体">（抗&mdash;</font><font face="Calibri">HBs</font><font face="宋体">）、</font><font face="Calibri">0.035</font><font face="宋体">（</font><font face="Calibri">HBeAg</font><font face="宋体">）、</font><font face="Calibri">1.083</font><font face="宋体">（抗&mdash;</font><font face="Calibri">HBe</font><font face="宋体">）、</font><font face="Calibri">1.065</font><font face="宋体">（抗&mdash;</font><font face="Calibri">HBc</font><font face="宋体">）、</font><font face="Calibri">0.045</font><font face="宋体">（抗</font><font face="Calibri">HBc-IgM</font><font face="宋体">）。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋体">生物梅里埃的</font>HIV<font face="宋体">试剂</font><font face="Calibri">NCx</font><font face="宋体">是</font><font face="Calibri">0.091</font><font face="宋体">。区分试剂</font><font face="Calibri">NCx</font><font face="宋体">值是不是合理的一个很简单的办法，只需比照大量阴性样本的实际读数就&ldquo;一目了然</font><font face="Calibri">&quot;</font><font face="宋体">啦！</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋体">（</font>2<font face="宋体">）阴性对照分为两类</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">&nbsp;ELISA<font face="宋体">试剂盒一是受检人血清中并不富含或办法学灵敏度未能检出的待检物的基准水平，而且结果判断值（</font><font face="Calibri">Cut off</font><font face="宋体">）核算式中决议&ldquo;是</font><font face="Calibri">&quot;</font><font face="宋体">（阳性）、或&ldquo;否</font><font face="Calibri">&quot;</font><font face="宋体">（阴性）的仅有可变值。阴性对照值高，致使假阴性；反之，</font><font face="Calibri">ELISA</font><font face="宋体">试剂盒致使假阳性。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋体">二是阴性对照设置，在办法学上请求参加指示性定量规范品，</font>ELISA<font face="宋体">试剂盒如中和剂</font><font face="Calibri">HBeAg</font><font face="宋体">，定量</font><font face="Calibri">HBcAg</font><font face="宋体">等，用以检查抗&mdash;</font><font face="Calibri">HBe</font><font face="宋体">、抗&mdash;</font><font face="Calibri">HBc</font><font face="宋体">。或许，选择代表平均水平的正常人血清用作阴性对照，</font><font face="Calibri">ELISA</font><font face="宋体">试剂盒如检查抗&mdash;</font><font face="Calibri">HBc IgM</font><font face="宋体">。此类</font><font face="Calibri">Cut off </font><font face="宋体">值核算时，大都均包含阴性和阳性对照值</font><font face="Calibri">2</font><font face="宋体">个可变值。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>