ELISA试验“温育”操作是不是只能在37℃条件下完结

<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋体">温育是</font><strong>ELISA<font face="宋体">试剂盒</font></strong><font face="宋体">测定中影响测定胜败最为关键的一个要素。</font><font face="Calibri">ELISA</font><font face="宋体">实验作为一种固相免疫测定，抗原抗体的联络反应在固相上进行，要使液相中的抗原或抗体与固相上的特异抗体或抗原彻底联络，必须在一定的温度条件下反应一定的时间。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋体">温育所需时间与温度成反比，即温度越高，则所需时间相对较短。在</font>ELISA<font face="宋体">中一般有两次抗原抗体反应，即加标本和加酶联络物后。抗原抗体反应的完结需要有一定的温度和时间，这一保温进程称为温育</font><font face="Calibri">(incubation)</font><font face="宋体">，也被称之为孵育。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">这是一个逐渐平衡的进程，因而需经涣散才华到达反应的结尾。在这今后参加的酶符号抗体与固相抗原的联络也相同如此。</span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><strong><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">ELISA<font face="宋体">试剂盒</font></span></strong><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋体">温育常选用的温度有</font><font face="Calibri">43</font><font face="宋体">℃、</font><font face="Calibri">37</font><font face="宋体">℃、室温文</font><font face="Calibri">4</font><font face="宋体">℃（冰箱温度）等。</font><font face="Calibri">37</font><font face="宋体">℃是实验室中常用的保温温度，也是大多数抗原抗体联络的适宜温度。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋体">实验标明，两次抗原抗体反应一般在</font>37<font face="宋体">℃经</font><font face="Calibri">1-2</font><font face="宋体">小时，产物的生成可达高峰。为加快反应，可前进反应的温度，</font><strong><font face="Calibri">ELISA</font><font face="宋体">试剂盒</font></strong><font face="宋体">有些实验在</font><font face="Calibri">43</font><font face="宋体">℃进行，但不宜选用更高的温度。抗原抗体反应</font><font face="Calibri">4</font><font face="宋体">℃更为彻底，在放射免疫测定中多使反应在冰箱中放置一晚上，以构成最多的堆积。但因所需时间太长，在</font><font face="Calibri">ELISA</font><font face="宋体">中一般不予选用。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>