ELISA试剂盒标本保存知多少

<p class="MsoNormal"><strong><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">ELISA<font face="宋体">试剂盒</font></span></strong><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋体">标本保留，在</font><font face="Calibri">ELISA</font><font face="宋体">试剂盒试验中是非常重要的，常有</font><font face="Calibri">ELISA</font><font face="宋体">操作员反映在标本保留时呈现溶血、凝聚不全、受细菌污染等景象发生，我公司技术员特对于有关常见问题做出总结。</font></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋体">下面咱们就来看看今日的内容：搞定</font><font face="Calibri">ELISA</font><font face="宋体">试剂盒办法我有妙招！</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="color: rgb(255, 0, 0);"><strong><span style="font-family: 宋体; font-size: 9pt;">一、标本保留不当</span></strong></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋体">在冰箱中保留过久的标本，血清中</font>IgG<font face="宋体">可聚组成多聚体、</font><font face="Calibri">AFP</font><font face="宋体">可构成二聚体，在间接法</font><font face="Calibri">ELISA</font><font face="宋体">测定中会致使本底过深、乃至构成假阳性；标本放置时刻过长（如一天以上），有时抗原或抗体免疫活性减弱，亦可呈现假阴性。为战胜上述搅扰，</font><font face="Calibri">ELISA</font><font face="宋体">测定的血清标本宜为新鲜收集；如不能当即测定，</font><font face="Calibri">5</font><font face="宋体">天内测定的血清标本可存放于</font><font face="Calibri">4</font><font face="宋体">℃，</font><font face="Calibri">1</font><font face="宋体">周后测定的血清标本应低温冻存；冻存后融解的标本，蛋白质局部浓缩，散布不均，应充沛混合后再测定，但混匀时应轻柔，不行激烈振动。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="color: rgb(255, 0, 0);"><strong><span style="font-family: 宋体; font-size: 9pt;">二、标本溶血</span></strong></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">ELISA<font face="宋体">试剂盒因为各种人为原因致使的标本溶血，均可因红细胞损坏溶解时释放出很多具有过氧化物酶活性的血红蛋白，在以辣根过氧化物酶为符号的</font><font face="Calibri">ELISA</font><font face="宋体">测定中，会致使非特异性显色，</font><font face="Calibri">ELISA</font><font face="宋体">试剂盒搅扰测定成果。为战胜上述搅扰效果，标本收集时有必要留意防止溶血。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="color: rgb(255, 0, 0);"><strong><span style="font-family: 宋体; font-size: 9pt;">三、标本凝聚不全</span></strong></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋体">在没有促凝剂和抗凝剂存在的状况下，正常血液收集后</font>1/2~2h<font face="宋体">开端凝结，</font><font face="Calibri">18~24h</font><font face="宋体">彻底凝结。临床查验工作中，有时为了争取时刻迅速检测，常在血液还未开端凝结时即强行离心别离血清，此刻的血清中仍残留部分纤维蛋白原，在</font><font face="Calibri">ELISA</font><font face="宋体">测定过程中能够构成肉眼可见的纤维蛋白块，易构成假阳性成果；这类状况于次日复查时因血凝已彻底，血清中不再有纤维蛋白原存在，故复查成果变为阴性。为防止上述搅扰效果，解决的办法最好是血液标本收集后有必要使其充沛凝结后再别离血清，或标本收集时用带别离胶的采血管或于采血管中参加适当的促凝剂。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="color: rgb(255, 0, 0);"><strong><span style="font-family: 宋体; font-size: 9pt;">四、标本管中添加物质的影响</span></strong></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋体">抗凝剂、酶抑制剂及迅速别离血清的别离胶等均对</font>ELISA<font face="宋体">测定有一定搅扰效果。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="color: rgb(255, 0, 0);"><strong><span style="font-family: 宋体; font-size: 9pt;">五、标本受细菌污染</span></strong></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">因菌体中可能富含内源性辣根过氧化物酶，因而，被细菌污染的标本同溶血标本一样，亦可产生非特异性显色而搅扰测定成果。</span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋体">做好了</font> <strong>ELISA<font face="宋体">试剂盒</font></strong><font face="宋体">要素和操作要素以后，保证检测成果的准确性就简单的多了。我公司试剂盒供给全程辅导服务，为您的试验担任究竟！欢迎您来电咨询我司业务员。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>