ELISA试剂盒的应注意防护

<p class="MsoNormal"><strong><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">ELISA<font face="宋体">试剂盒</font></span></strong><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋体">是固相夹心法酶联免疫吸附实验（</font><font face="Calibri">ELISA</font><font face="宋体">）</font><font face="Calibri">.</font><font face="宋体">已知浓度的标准品、未知浓度的样品加入微孔酶标板内进行检测。先将生物素标记的抗体同时温育。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋体">洗涤后，</font><strong>ELISA<font face="宋体">试剂盒</font></strong><font face="宋体">加入亲和素标记过的</font><font face="Calibri">HRP</font><font face="宋体">。再经过温育和洗涤，去除未结合的酶结合物，然后加入底物</font><font face="Calibri">A</font><font face="宋体">、</font><font face="Calibri">B</font><font face="宋体">，和酶结合物同时作用。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋体">产生颜色：</font>ELISA<font face="宋体">试剂盒颜色的深浅和样品中的浓度呈比例关系。</font><font face="Calibri">ELSIA</font><font face="宋体">操作步骤复杂，影响反应因素较多，特别是固相载体的包被难达到各个体之间的一致</font><font face="Calibri">.</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋体">因此在定量测定中，</font> ELISA<font face="宋体">试剂盒每批测试均须用一系列不同浓度的参考标准品在相同的条件下制作标准曲线。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋体">测定大分子量物质的夹心法</font>ELISA<font face="宋体">试剂盒，标准曲线的范围一般较宽，曲线最高点的吸光度可接近</font><font face="Calibri">2.0</font><font face="宋体">，绘制时常用半对数纸，以检测物的浓度为横坐标，</font><font face="Calibri">ELISA</font><font face="宋体">试剂盒以吸光度为纵坐标，将各浓度的值逐点连接，所得曲线一般呈</font><font face="Calibri">S</font><font face="宋体">形，其头、尾部曲线趋于平坦，中央较呈直线的部分是最理想的检测区域。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><strong><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">ELISA<font face="宋体">试剂盒</font></span></strong><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋体">酶的底物及供氢体的选择：对供氢体的选择要求是价廉、安全、有明显地显色反应，而本身无色。有些供氢体（如</font><font face="Calibri">OPD</font><font face="宋体">等）有潜在的致癌作用。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋体">有条件者应使用不致癌、灵敏度高的供氢体，如</font>TMB<font face="宋体">和</font><font face="Calibri">ABTS</font><font face="宋体">是目前较为满意的供氢体。</font><font face="Calibri">ELISA</font><font face="宋体">试剂盒底物作用一段时间后，应加入强酸或强碱以终止反应。通常底物作用时间，以</font><font face="Calibri">10-30</font><font face="宋体">分钟为宜。底物使用液必须新鲜配制，尤其是</font><font face="Calibri">H2O2</font><font face="宋体">在临用前加入。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>